Fur Protein Regulates the Motility of Avian Pathogenic Escherichia coli AE17 Through Promoter Regions of the Flagella Key Genes flhD.
Fur Protein Regulates the Motility of Avian Pathogenic Escherichia coli AE17 Through Promoter Regions of the Flagella Key Genes flhD.
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DOI：
10.3389/fvets.2022.854916
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发表时间：
2022
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
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禽致病性大肠杆菌（Avian pathogenic Escherichia coli，APEC）是引起禽类多种疾病的重要病原。它是家禽局部和全身感染的罪魁祸首，严重阻碍了家禽业的发展，并对公共卫生构成潜在风险。铁吸收调节蛋白Fur及其负调控的非编码RNA RyhB是细菌铁吸收的重要因素，但细菌毒力基因的调控在不同细菌中差异很大。我们发现，皮草对APEC的流动性非常重要。Fur和RyhB的表达在APEC中受到广泛调节，并且RyhB的表达也受到Fur的负调节。转录组学分析表明，由毛皮显著差异调节的基因与细胞运动有关，包括纤毛或鞭毛依赖的细胞运动。为了验证这些结果，我们通过测量细菌菌落的直径来检查皮毛敲除对细胞运动的影响。与RNA测序结果一致，AE17Δfur的移动性较野生型明显降低，几乎已经丧失了移动能力。使用电泳迁移率测定，我们证实，毛皮蛋白直接结合到关键的鞭毛相关基因flhD的启动子区，从而影响APEC鞭毛的组装和合成。这项研究扩展了我们对APEC基因调控的理解。
Avian pathogenic Escherichia coli (APEC) is an important pathogen causing several diseases in birds. It is responsible for local and systemic infections in poultry, seriously impeding the development of the poultry industry, and poses a potential risk to public health. The iron absorption regulatory protein Fur and the noncoding RNA, RyhB, that it negatively regulates are important factors in bacterial iron uptake, but the regulation of bacterial virulence genes varies greatly among different bacteria. We found that Fur is very important for the mobility of APEC. The expression of fur and RyhB is extensively regulated in APEC, and RyhB expression is also negatively regulated by Fur. A transcriptomic analysis showed that the genes significantly differentially regulated by Fur are related to cell movement, including pilus- or flagellum-dependent cell motility. To verify these results, we examined the effects of fur knockdown on cell movement by measuring the diameter of the bacteria colonies. Consistent with the RNA sequencing results, the mobility of AE17Δfur was significantly reduced compared with that of the wild type, and it had almost lost its ability to move. Using an electrophoretic mobility assay, we confirmed that the Fur protein directly binds to the promoter region of the key flagellum-related gene flhD, thereby affecting the assembly and synthesis of the APEC flagellum. This study extends our understanding of gene regulation in APEC.
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影响因子：
6.7
作者：
Rossez Y;Wolfson EB;Holmes A;Gally DL;Holden NJ
通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
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通讯作者：
Wanner, BL