Isolation, sequence identification and tissue expression profile of a novel ribokinase gene (RBKS) from Chinese Banna mini-pig inbred line (BMI)
Isolation, sequence identification and tissue expression profile of a novel ribokinase gene (RBKS) from Chinese Banna mini-pig inbred line (BMI)
复制标题
中国版纳小型猪自交系（BMI）新核糖激酶基因（RBKS）的分离、序列鉴定及组织表达谱
DOI：
10.5897/ajb11.2885
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发表时间：
2011-01
期刊：
影响因子：
--
通讯作者：
Xiao Heng
中科院分区：
文献类型：
--
作者：
Huo Hailong;Liu Lixian;Zeng Yangzhi;Xiao Heng
根据牛或其他哺乳动物的保守序列信息和已知的高度同源的猪EST序列，采用逆转录-聚合酶链式反应(RT-PCR)扩增版纳小型猪近交系(BMI)核蛋白激酶基因(RBKS)的完整表达序列标签(CDS)。将该新基因登录到NCBI数据库中，编号为JF944892。序列分析表明，BMI RBKS编码的蛋白由323个氨基酸组成，与牛(99%)、马(99%)、猩猩(99%)、人(89%)、猴(89%)、大鼠(88%)和小鼠(80%)等7种核蛋白有很高的同源性。系统进化树分析表明，BMI RBKS基因与牛和马的RBKS基因的亲缘关系比与猩猩、人、猴、大鼠和小鼠的亲缘关系更近。RT-PCR分析表明，BMI RBKS基因在卵巢和肺组织中高表达，在脾、神经纤维、大肠和间脑中中等表达，在心脏、皮肤、肌肉、小肠、中脑、肾脏和脂肪中弱表达，在其他五个组织中几乎不表达。在BMI RBKS基因的CDS中预测了4个microRNA靶点，为进一步研究该基因奠定了基础。同源模建的RBKS的三维结构与人核糖激酶(2fv7)相似。我们的实验将为进一步深入了解这种猪基因奠定基础。关键词：版纳小型猪近交系，猪，核蛋白基因，组织表达分析，同源建模
The complete expressed sequence tag (CDS) sequence of Banna mini-pig inbred line (BMI) ribokinase gene (RBKS) was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to accession number JF944892. Sequence analysis revealed that the BMI RBKS encodes a protein of 323 amino acids that has high homology with the ribokinase proteins of seven species: cattle (99%), horse (99%), orangutan (99%), human (89%), monkey (89%), rat (88%) and mouse (80%). The phylogenetic tree analysis revealed that the BMI RBKS gene has a closer genetic relationship with the RBKS genes of bovine and horse than with those of orangutan, human, monkey, rat and mouse. Analysis by RT-PCR showed that BMI RBKS gene was over-expressed in ovary and lung, moderately expressed in spleen, nerve fiber, large intestine and diencephalon, weakly expressed in heart, skin, muscle, small intestine, midbrain, kidney and fat, while almost silent in other five tissues. Four microRNA target sites were predicted in the CDS of BMI RBKS mRNA for further study of this gene in the future. The 3D structure of the RBKS by homology modeling was similar to that of human ribokinase (2fv7). Our experiment will establish a foundation for further insight into this swine gene. Key words : Banna mini-pig inbred line (BMI), pig, ribokinase gene (RBKS), tissue expression analysis, homology modeling
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DOI：
10.1073/pnas.1630797100
发表时间：
2003-08-19
影响因子：
11.1
作者：
Zeng, Y;Yi, R;Cullen, BR
通讯作者：
Cullen, BR
影响因子：
5.8
作者：
Möller, S;Croning, MDR;Apweiler, R
通讯作者：
Apweiler, R
影响因子：
3.2
作者：
WU, LF;REIZER, A;SAIER, MH
通讯作者：
SAIER, MH
DOI：
10.1016/s0925-4439(98)00093-3
发表时间：
1999-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
C. Salerno;P. D'eufemia;R. Finocchiaro;M. Celli;A. Spalice;P. Iannetti;C. Crifó;O. Giardini
通讯作者：
C. Salerno;P. D'eufemia;R. Finocchiaro;M. Celli;A. Spalice;P. Iannetti;C. Crifó;O. Giardini
影响因子：
5.6
作者：
Sigrell, JA;Cameron, AD;Mowbray, SL
通讯作者：
Mowbray, SL