Comparative Analysis of Proteins Involved in Energy Metabolism and Protein Processing in Pyropia haitanensis at Different Salinity Levels
Comparative Analysis of Proteins Involved in Energy Metabolism and Protein Processing in Pyropia haitanensis at Different Salinity Levels
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不同盐度坛紫菜能量代谢和蛋白质加工相关蛋白质的比较分析
DOI：
10.3389/fmars.2020.00415
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发表时间：
2020-06
影响因子：
3.7
通讯作者：
谢潮添
中科院分区：
文献类型：
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作者：
闻健;王文磊;许凯;纪德华;徐燕;陈昌生;谢潮添
土壤盐渍化的加剧已成为限制农作物产量和品质的重要因素。坛Pyropia haitanensis生长在潮间带，具有很强的耐盐性。生物体对压力的反应机制与能量代谢和蛋白质加工有关。然而，高盐胁迫对坛紫菜叶状体能量代谢和蛋白质合成的影响尚未见报道。因此，本研究旨在应用数据独立采集（DIA）技术，分析坛紫菜在100%和110%盐度胁迫下，与能量代谢和蛋白质加工相关的蛋白质丰度变化的差异。对高盐胁迫下藻类能量代谢的研究表明，磷酸果糖激酶丰度的降低抑制了糖酵解途径。然而，磷酸丙糖异构酶水平的增加和磷酸戊糖途径的增强可以最大限度地减少这种抑制作用，同时还为柠檬酸循环提供底物，并为高盐胁迫条件下的生理和生化藻类反应提供能量。关于与内质网相关的蛋白质加工，CNX、转运蛋白COP II、热休克蛋白、蛋白质二硫键异构酶和与内质网质量控制相关的其他蛋白质的丰度的增加将确保蛋白质在高盐胁迫下在藻类中合成。内质网相关降解识别蛋白BiP和转运蛋白Sec 61以及泛素化降解系统相关蛋白水平的增加将维持藻类中蛋白质折叠和清除之间的动态平衡。此外，藻类对100%的盐度具有耐受性，但110%的盐度对藻类具有半致死性。柠檬酸合成酶、6-磷酸葡萄糖脱氢酶、蛋白质二硫键异构酶等相关蛋白的含量在100%盐胁迫下增加，而在110%盐胁迫下没有增加。因此，坛紫菜叶状体在100%盐胁迫下可能会诱导代谢活动，以满足生理生化反应的需要。此外，可以稳定蛋白质加工和合成以确保完成必要的应激反应。本研究结果为植物抗逆机制的研究和耐盐新品种的培育提供了重要依据。
The worsening of soil salinization has become an important factor limiting crop yield and quality. Pyropia haitanensis grows in the intertidal zone and is highly salt tolerant. The response mechanism of organisms exposed to stress is linked to energy metabolism and protein processing. However, the effect of hypersaline stress on the energy metabolism and protein synthesis of the P haitanensis thallus has not been reported. Therefore, the objective of this study was to apply data-independent acquisition (DIA) technology to analyze the differences in the changes to the abundance of proteins related to energy metabolism and protein processing in P haitanensis thalli exposed to salinity stress (100%) and semilethal salinity (110%). The examination of energy metabolism in algae under hypersaline stress revealed the decreased abundance of phosphofructokinase inhibits the glycolytic pathway. However, increases in triosephosphate isomerase levels and the enhancement of the pentose phosphate pathway minimize this inhibitory effect, while also providing substrates for the citric acid cycle as well as energy for physiological and biochemical algal reactions under hypersaline stress conditions. Regarding the protein processing related to the endoplasmic reticulum, increases in the abundance of CNX, the transporter COP II, heat shock proteins, protein disulfide isomerase, and other proteins related to endoplasmic reticulum quality control will ensure proteins are synthesized in algae under hypersaline stress. Increases in the levels of endoplasmic reticulum-associated degradation recognition protein BiP and transporter Sec61 as well as the ubiquitination degradation system-related proteins will maintain the dynamic balance between protein folding and clearance in algae. Moreover, algae are tolerant to 100% salinity, but 110% salinity is semilethal to algae. The abundances of citrate synthase, 6-phosphoglucose dehydrogenase, protein disulfide isomerase, and other related proteins increased under 100% salinity stress, but not under 110% salinity stress. Accordingly, in response to 100% salinity stress, P. haitanensis thalli may induce metabolic activities to satisfy the requirements of physiological and biochemical reactions. Furthermore, protein processing and synthesis may be stabilized to ensure necessary stress reactions are completed. The results of this study provide data potentially useful for investigating plant stress resistance mechanisms and for cultivating new salt-tolerant varieties.
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