Tdrd6 is required for spermiogenesis, chromatoid body architecture, and regulation of miRNA expression.
Tdrd6 is required for spermiogenesis, chromatoid body architecture, and regulation of miRNA expression.
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DOI：
10.1016/j.cub.2009.02.047
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发表时间：
2009-04-28
期刊：
影响因子：
--
通讯作者：
Jessberger R
中科院分区：
文献类型：
--
作者：
Vasileva A;Tiedau D;Firooznia A;Müller-Reichert T;Jessberger R
染色质体是精细胞的细胞器，具有RNA储存和小RNA加工的功能，但其功能尚不清楚。CB成分包括Mili、Miwi和tudor结构域蛋白，如Tdrd6，它们对CB结构和功能的贡献尚不清楚。我们确定了Tdrd6在配子体发育阶段和雄性特异性的表达和定位，确定了减数分裂I后c端截断形式占主导地位，并证明了Tdrd6与CB组分Mili和Miwi的直接物理相互作用。在Tdrd6−/−小鼠中，从圆形精子细胞发育为细长精子细胞是不存在的。它们的圆形精子携带“幽灵”CBs，其结构被严重破坏。Mael， Miwi和Mvh不定位于tdrd6缺陷的CBs，但反转录转座子没有明显激活。然而，超过50种mirna在Tdrd6−/−睾丸中更为丰富，作为典型的pre-和pri- mirna。我们得出结论，Tdrd6对精子发生、CB结构以及成熟和前体miRNA的正确表达至关重要。
Chromatoid bodies (CBs) are characteristic spermatid organelles, which were suggested to function in RNA storage and small RNA processing, but whose functions remain largely unknown. CB components include Mili, Miwi, and Tudor-domain proteins such as Tdrd6, whose contribution to CB structure and function is elusive. We determined gametogenesis stage- and male-specific expression and localization of Tdrd6, identified a C-terminally truncated form as predominant after meiosis I, and demonstrate direct physical interaction of Tdrd6 with the CB components Mili and Miwi. Development from round into elongated spermatids is abrogated in Tdrd6−/− mice. Their round spermatids bear “ghost” CBs, whose architecture is greatly disrupted. Mael, Miwi, and Mvh do not localize to the Tdrd6-deficient CBs, but retrotransposons are not significantly activated. However, more than 50 miRNAs are more abundant in Tdrd6−/− testes, as are exemplary pre- and pri-miRNAs. We conclude that Tdrd6 is essential for spermiogenesis, for CB structure, and for proper mature and precursor miRNA expression.
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