Effects of Epigallocatechin-3-Gallate on Autophagic Lipolysis in Adipocytes.
Effects of Epigallocatechin-3-Gallate on Autophagic Lipolysis in Adipocytes.
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DOI：
10.3390/nu9070680
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发表时间：
2017-06-30
期刊：
影响因子：
5.9
通讯作者：
Lee YH
中科院分区：
文献类型：
--
作者：
Kim SN;Kwon HJ;Akindehin S;Jeong HW;Lee YH
以前的研究证明了绿色茶对减肥的影响;然而，绿色茶诱导的脂肪细胞功能调节尚未完全了解。在这里，我们研究了主要的绿色茶植物化学物质，表没食子儿茶素-3-没食子酸酯（EGCG）对甘油三酯含量，脂解，线粒体功能和自噬的影响，在体外分化的脂肪细胞从C3 H10 T1/2细胞和永生化前脂肪细胞。与营养饥饿状态相比，EGCG使脂肪细胞中的三甘油含量显著降低了25%。表没食子儿茶素没食子酸酯不影响蛋白激酶A信号或棕色脂肪细胞标记物在脂肪细胞中的表达;然而，表没食子酸酯增加自噬，如通过自噬通量分析和LC 3B、ATG 7和Beclin 1的免疫印迹分析所测量的。与对照组相比，表没食子酸没食子酸酯处理使线粒体膜电位降低56.8%，细胞内ATP水平降低49.1%。虽然哺乳动物靶雷帕霉素信号转导没有上调表没食子儿茶素没食子酸酯处理，表没食子儿茶素没食子酸酯处理诱导AMP激活的蛋白激酶磷酸化，表明能量耗尽状态。此外，EGCG增加了RAB 7和脂滴之间的关联，表明脂肪吞噬被激活。最后，Rab 7的敲除减弱了EGCG依赖的脂质含量的降低。总的来说，这些结果表明，EGCG上调脂肪细胞中的自噬脂解，支持EGCG作为热量限制模拟物预防肥胖和肥胖相关代谢疾病的治疗潜力。
Previous studies demonstrated effects of green tea on weight loss; however, green tea-induced modulation of adipocyte function is not fully understood. Here, we investigated effects of the major green tea phytochemical, epigallocatechin-3-gallate (EGCG) on triglyceride contents, lipolysis, mitochondrial function, and autophagy, in adipocytes differentiated from C3H10T1/2 cells and immortalized pre-adipocytes in vitro. EGCG reduced the triglycerol content significantly in adipocytes by 25%, comparable to the nutrient starvation state. EGCG did not affect protein kinase A signaling or brown adipocyte marker expression in adipocytes; however, EGCG increased autophagy, as measured by autophagy flux analysis and immunoblot analysis of LC3B, ATG7, and Beclin1. EGCG treatment reduced mitochondrial membrane potential by 56.8% and intracellular ATP levels by 49.1% compared to controls. Although mammalian target of rapamycin signaling was not upregulated by EGCG treatment, EGCG treatment induced AMP-activated protein kinase phosphorylation, indicating an energy-depleted state. In addition, EGCG increased the association between RAB7 and lipid droplets, suggesting that lipophagy was activated. Finally, knockdown of Rab7 attenuated the EGCG-dependent reduction in lipid contents. Collectively, these results indicated that EGCG upregulated autophagic lipolysis in adipocytes, supporting the therapeutic potential of EGCG as a caloric restriction mimetic to prevent obesity and obesity-related metabolic diseases.
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影响因子：
5.9
作者：
Tang W;Song H;Cai W;Shen X
通讯作者：
Shen X
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作者：
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作者：
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通讯作者：
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作者：
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3.6
作者：
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通讯作者：
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