Botulinum neurotoxin A blocks synaptic vesicle exocytosis but not endocytosis at the nerve terminal.
Botulinum neurotoxin A blocks synaptic vesicle exocytosis but not endocytosis at the nerve terminal.
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肉毒杆菌神经毒素A阻断了神经末端的突触囊泡胞吐作用，但没有内吞作用。
DOI：
10.1083/jcb.147.6.1249
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发表时间：
1999-12-13
期刊：
影响因子：
--
通讯作者：
Williamson LC
中科院分区：
文献类型：
--
作者：
Neale EA;Bowers LM;Jia M;Bateman KE;Williamson LC
The supply of synaptic vesicles in the nerve terminal is maintained by a temporally linked balance of exo- and endocytosis. Tetanus and botulinum neurotoxins block neurotransmitter release by the enzymatic cleavage of proteins identified as critical for synaptic vesicle exocytosis. We show here that botulinum neurotoxin A is unique in that the toxin-induced block in exocytosis does not arrest vesicle membrane endocytosis. In the murine spinal cord, cell cultures exposed to botulinum neurotoxin A, neither K+-evoked neurotransmitter release nor synaptic currents can be detected, twice the ordinary number of synaptic vesicles are docked at the synaptic active zone, and its protein substrate is cleaved, which is similar to observations with tetanus and other botulinal neurotoxins. In marked contrast, K+ depolarization, in the presence of Ca2+, triggers the endocytosis of the vesicle membrane in botulinum neurotoxin A–blocked cultures as evidenced by FM1-43 staining of synaptic terminals and uptake of HRP into synaptic vesicles. These experiments are the first demonstration that botulinum neurotoxin A uncouples vesicle exo- from endocytosis, and provide evidence that Ca2+ is required for synaptic vesicle membrane retrieval.
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DOI：
10.1083/jcb.57.2.499
发表时间：
1973-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ceccarelli B;Hurlbut WP;Mauro A
通讯作者：
Mauro A
影响因子：
7.8
作者：
HEUSER, JE;REESE, TS
通讯作者：
REESE, TS
影响因子：
56.9
作者：
BETZ, WJ;BEWICK, GS
通讯作者：
BEWICK, GS
影响因子：
16.2
作者：
BROADIE, K;PROKOP, A;SWEENEY, ST
通讯作者：
SWEENEY, ST
影响因子：
64.8
作者：
Klingauf, J;Kavalali, ET;Tsien, RW
通讯作者：
Tsien, RW