Long-lasting intrinsic optical changes observed in the neurointermediate lobe of the mouse pituitary reflect volume changes in cells of the pars intermedia.
Long-lasting intrinsic optical changes observed in the neurointermediate lobe of the mouse pituitary reflect volume changes in cells of the pars intermedia.
复制标题
在小鼠垂体神经中间叶中观察到的持久内在光学变化反映了中间部细胞的体积变化。
DOI：
10.1159/000314619
复制
发表时间：
2010
影响因子：
4.1
通讯作者：
Salzberg,BM
中科院分区：
文献类型：
--
作者：
Kosterin,P;Obaid,AL;Salzberg,BM
背景/目的电刺激小鼠脑垂体漏斗柄后，可在其神经中间叶观察到复杂的内在光学变化（光散射）。我们的实验室先前已经确定了光散射信号中的三个不同阶段：对动作电位刺激的两个快速反应和长时间恢复。快速光散射信号，仅限于神经垂体部分（垂体后叶）的神经中间叶，包括E波和S波，分别反映兴奋和分泌。E波具有动作电位的近似形状，包括电压和电流相关成分，并且不依赖于Ca 2+内流。S波与Ca 2+内流和胞吐有关。光散射信号的缓慢恢复阶段，我们指定的R-波，是不太好的characterized.MethodsUsing高时间分辨率的光散射测量，我们监测内在的光学变化，在神经中间叶的小鼠脑垂体。在测量过程中的药理学干预employed.ResultsThe数据提供光学和药理学证据表明，R-波，其中包括来自垂体后叶的信号，以及从中间部，反映在中间部细胞的体积变化后，施加到漏斗柄的一系列刺激。这些体积的变化被阻断的GABA受体拮抗剂荷包牡丹碱和印防己毒素，并模仿直接应用的GABA在没有电stimulation.ConclusionsThese结果强调中央GABA能投射到neurointermediate叶的重要性，和潜在的作用，GABA在影响激素释放的中间部。
Background/AimsComplex intrinsic optical changes (light scattering) are readily observed in the neurointermediate lobe of the mouse pituitary gland following electrical stimulation of the infundibular stalk. Our laboratory has previously identified three distinct phases within the light scattering signal: two rapid responses to action potential stimulation and a long duration recovery. The rapid light scattering signals, restricted to the neurohypophysial portion (posterior pituitary) of the neurointermediate lobe, consist of an E-wave and an S-wave that reflect excitation and secretion, respectively. The E-wave has the approximate shape of the action potential and includes voltage-and current-related components and is independent of Ca 2+ entry. The S-wave is related to Ca 2+ entry and exocytosis. The slow recovery phase of the light scattering signal, which we designated the R-wave, is less well characterized.MethodsUsing high temporal resolution light scattering measurements, we monitored intrinsic optical changes in the neurointermediate lobe of the mouse pituitary gland. Pharmacological interventions during the measurements were employed.ResultsThe data presented here provide optical and pharmacological evidence suggesting that the R-wave, which comprises signals from the posterior pituitary as well as from the pars intermedia, mirrors volume changes in pars intermedia cells following a train of stimuli applied to the infundibular stalk. These volume changes were blocked by the GABA-receptor antagonists bicuculline and picrotoxin, and were mimicked by direct application of GABA in the absence of electrical stimulation.ConclusionsThese results emphasize the importance of central GABAergic projections into the neurointermediate lobe, and the potential role of GABA in effecting hormone release from the pars intermedia.
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影响因子：
14.9
作者：
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作者：
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DOI：
--
发表时间：
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期刊：
影响因子：
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作者：
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DOI：
--
发表时间：
1986
期刊：
影响因子：
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作者：
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