Effect of a stilbene glycoside-rich extract from Polygoni Multiflori Radix on experimental non-alcoholic fatty liver disease based on principal component and orthogonal partial least squares discriminant analysis.
Effect of a stilbene glycoside-rich extract from Polygoni Multiflori Radix on experimental non-alcoholic fatty liver disease based on principal component and orthogonal partial least squares discriminant analysis.
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基于主成分和正交偏最小二乘判别分析的何首乌富含二苯乙烯苷提取物对实验性非酒精性脂肪肝的影响
DOI：
10.3892/etm.2017.5197
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发表时间：
2017-11
影响因子：
2.7
通讯作者：
Lv G
中科院分区：
文献类型：
--
作者：
Lou Z;Xia B;Su J;Yu J;Yan M;Huang Y;Lv G
黄芪是一种传统中药，临床上用于支持肝脏和肾脏的功能，并治疗高脂血症。在以往的研究中，从黄芪中分离得到一个有效部位，富含2，3，5，4 ′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷（TSG），具有降血脂活性。本研究旨在系统地评估该组分对非酒精性脂肪性肝病（NAFLD）的影响。采用高脂饲料喂养Sprague-Dawley大鼠18周，建立大鼠非酒精性脂肪肝模型。苏木精-伊红染色用于肝组织病理学分析。此外，酶活性，脂质代谢，炎症因子和胰岛素抵抗指数测定使用全自动血液生化分析仪和ELISA。采用逆转录-定量聚合酶链反应（RT-PCR）和蛋白质印迹法（Western blot）检测细胞色素P450 2 E1（CYP 2 E1）和过氧化物酶体增殖物激活受体α（PPARα）的mRNA和蛋白表达。采用主成分分析和正交偏最小二乘判别分析对数据进行分析。结果表明，富TSG-rich Fraction（TSGP）能显著降低血清总胆固醇和甘油三酯水平，降低肝脏游离脂肪酸、CYP 2 E1 mRNA和丙二醛水平，并能减轻肝脏肿大和脂肪变性。此外，它还能上调肝组织中PPARα mRNA的表达。结果表明，TSGP对NAFLD具有保护作用，其机制可能与通过调节PPARα和CYP 2 E1介导的途径增强抗脂质过氧化能力有关。
Polygoni Multiflori Radix is a traditional Chinese medicine used clinically to support the functions of the liver and kidneys and to treatment hyperlipidemia. In previous studies, an effective fraction, rich in 2,3,5,4′-tetrahydroxy stilbene-2-O-β-D-glucoside (TSG), was separated from Polygoni Multiflori Radix and demonstrated hypolipidemic activity. The present study aimed to systematically assess the effect of this fraction on non-alcoholic fatty liver disease (NAFLD). A NAFLD model was established by feeding Sprague-Dawley rats a high-fat diet with 10% fructose solution for 18 weeks. Hematoxylin and eosin staining was applied for hepatic histopathological analysis. In addition, enzyme activities, lipid metabolism, inflammatory factors and insulin resistance indices were measured using a fully automatic blood biochemistry analyser and ELISA. Furthermore, cytochrome P450 2E1 (CYP2E1) and peroxisome proliferator-activated receptor α (PPARα) mRNA and protein expression were evaluated using reverse transcription-quantitative polymerase chain reaction and western blot analysis. Principal component analysis and orthogonal partial least squares discriminant analysis were used to analyse the data. The results revealed that the TSG-rich fraction (TSGP) significantly lowered the serum total cholesterol and triglyceride levels, and the liver free fatty acid, CYP2E1 mRNA and malondialdehyde levels, in addition to mitigating hepatic enlargement and alleviating liver steatosis. Furthermore, it upregulated PPARα mRNA expression in the liver tissue. The results indicated that TSGP exhibited a protective effect against NAFLD and the underlying mechanism may involve augmentation of anti-lipid peroxidation capacity via regulation of PPARα and CYP2E1-mediated pathways.
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影响因子：
4.5
作者：
Liu J;Han L;Zhu L;Yu Y
通讯作者：
Yu Y
影响因子：
8.8
作者：
Lv, LiShuang;Gu, XiaoHong;Ho, Chi-Tang
通讯作者：
Ho, Chi-Tang
影响因子：
5.4
作者：
Phung, Nghi;Pera, Natasha;George, Jacob
通讯作者：
George, Jacob
影响因子：
3.4
作者：
Lv, Guiyuan;Gu, Hui;Shan, Letian
通讯作者：
Shan, Letian
影响因子：
5.2
作者：
Heck DE;Shakarjian M;Kim HD;Laskin JD;Vetrano AM
通讯作者：
Vetrano AM