Selective Fos induction in hypothalamic orexin/hypocretin, but not melanin-concentrating hormone neurons, by a learned food-cue that stimulates feeding in sated rats.
Selective Fos induction in hypothalamic orexin/hypocretin, but not melanin-concentrating hormone neurons, by a learned food-cue that stimulates feeding in sated rats.
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DOI：
10.1016/j.neuroscience.2012.08.036
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发表时间：
2012-11-08
期刊：
影响因子：
3.3
通讯作者：
Reppucci, C. J.
中科院分区：
文献类型：
--
作者：
Petrovich, G. D.;Hobin, M. P.;Reppucci, C. J.
联想学习可以使来自环境的线索在没有生理饥饿的情况下刺激进食。学习线索如何与稳态调节系统整合尚不清楚。在这里，我们研究了潜在的机制是否涉及下丘脑食欲神经肽调节剂食欲素/下丘脑泌素（ORX）和黑色素浓集激素（MCH）。我们使用巴甫洛夫条件反射程序来训练食物限制的大鼠将一个离散的线索，一个音调，与不同于他们常规实验室食物饮食的食物颗粒联系起来。条件组（配对）中的大鼠在食物递送之前立即接受音调的呈现，而对照组（未配对）中的大鼠接受相同数量的音调和食物颗粒的随机呈现。调节后，在测试前，允许大鼠自由进食实验室食物至少10天。在测试时，在它们的饲养笼中向饱食的大鼠提供音调，然后允许一组消耗食物颗粒，而另一组不受干扰直到处死用于Fos诱导分析。在这种情况下，音调提示刺激食物消耗;配对组中的大鼠比未配对组中的大鼠消耗更大量的食物颗粒。为了检查Fos诱导，我们使用荧光免疫组织化学方法处理脑组织，用于联合检测Fos和表征ORX和MCH神经元。我们发现，与未配对条件相比，配对条件下ORX和Fos双标记神经元的百分比更大，特别是在穹窿周围区域。相比之下，在配对和未配对条件下都有很少的MCH神经元具有Fos诱导。因此，食物线索选择性地诱导ORX中的Fos，但不诱导MCH神经元中的Fos。这些结果表明，ORX在没有生理饥饿的情况下发生的线索诱导摄食中的作用。
Associative learning can enable cues from the environment to stimulate feeding in the absence of physiological hunger. How learned cues are integrated with the homeostatic regulatory system is unknown. Here we examined whether the underlying mechanism involves the hypothalamic orexigenic neuropeptide regulators orexin/hypocretin (ORX) and melanin-concentrating hormone (MCH). We used a Pavlovian conditioning procedure to train food-restricted rats to associate a discrete cue, a tone, with food pellets distinct from their regular lab chow diet. Rats in the conditioned group (Paired) received presentations of a tone immediately prior to food delivery, while the rats in the control group (Unpaired) received random presentations of the same number of tones and food pellets. After conditioning rats were allowed ad libitum access to lab chow for at least 10 days before testing. At test sated rats were presented with the tones in their home cages, and then one group was allowed to consume food pellets, while another group was left undisturbed until sacrifice for Fos induction analysis. The tone cue stimulated food consumption in this setting; rats in the Paired group consumed larger amounts of food pellets than rats in the Unpaired group. To examine Fos induction we processed the brain tissue using fluorescent immunohistochemistry methods for combined detection of Fos and characterization of ORX and MCH neurons. We found a greater percentage of ORX and Fos double-labeled neurons in the Paired compared to the Unpaired condition, specifically in the perifornical area. In contrast, there were very few MCH neurons with Fos induction in both the Paired and Unpaired conditions. Thus, the food-cue selectively induced Fos in ORX but not in MCH neurons. These results suggest a role for ORX in cue-induced feeding that occurs in the absence of physiological hunger.
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