Pig zona pellucida 2 (pZP2) protein does not participate in zona pellucida formation in transgenic mice.
Pig zona pellucida 2 (pZP2) protein does not participate in zona pellucida formation in transgenic mice.
复制标题
猪透明带 2 (pZP2) 蛋白不参与转基因小鼠透明带的形成。
作者：
A. Hasegawa;Nozomi Kanazawa;H. Sawai;S. Komori;K. Koyama
透明质酸是哺乳动物卵母细胞周围的细胞外基质，由三种或四种糖蛋白组成。众所周知，透明壳在受精过程中起着几个关键作用，但对其形成的了解甚少。本研究的目的是检测猪透明质酸糖蛋白2（pZP2）是否会与小鼠透明质酸组装。通过将编码pZP2的小基因置于小鼠ZP2启动子的下游来制备转基因构建体。结果表明，转基因蛋白在生长期卵母细胞中合成，但未掺入透明质酸中。此外，pZP2转基因不能挽救ZP2基因敲除的透明带缺陷小鼠的表型。这些结果表明，pZP2不参与小鼠透明质酸的形成和透明质酸是由其组成蛋白质在小鼠和猪之间的分子物种特异性的方式。
The zona pellucida, an extracellular matrix surrounding mammalian oocytes, is composed of three or four glycoproteins. It is well known that the zona pellucida plays several critical roles during fertilization, but there is little knowledge about its formation. The purpose of this study is to examine whether a pig zona pellucida glycoprotein 2 (pZP2) would assemble with mouse zona pellucida. A transgene construct was prepared by placing a minigene encoding pZP2 downstream from the promoter of mouse ZP2. The result showed that the transgenic protein was synthesized in growing oocytes but not incorporated into the zona pellucida. Furthermore, the pZP2 transgene did not rescue the phenotype in ZP2-knockout zona-deficient mice. These results indicate that pZP2 does not participate in mouse zona pellucida formation and the zona pellucida is constituted from its component proteins in a molecular species-specific manner between mice and pigs.
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DOI：
10.1073/pnas.0401600101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Jovine, L;Qi, HY;Wassarman, PM
通讯作者：
Wassarman, PM
影响因子：
4.6
作者：
T. Rankin;M. O'Brien;Eric Lee;K. Wigglesworth;J. Eppig;Jurrien Dean
通讯作者：
T. Rankin;M. O'Brien;Eric Lee;K. Wigglesworth;J. Eppig;Jurrien Dean
影响因子：
11.8
作者：
Rankin, TL;Coleman, JS;Dean, J
通讯作者：
Dean, J
一种针对小鼠透明带硫酸化糖蛋白 ZP2 的避孕肽疫苗。
影响因子：
3.6
作者：
Sun,W;Lou,YH;Dean,J;Tung,KS
通讯作者：
Tung,KS
影响因子：
3.6
作者：
ERBACH, GT;LAWITTS, JA;BIGGERS, JD
通讯作者：
BIGGERS, JD