DNA looping facilitates targeting of a chromatin remodeling enzyme.
DNA looping facilitates targeting of a chromatin remodeling enzyme.
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DNA循环有助于靶向染色质重塑酶。
DOI：
10.1016/j.molcel.2013.02.005
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发表时间：
2013-04-11
期刊：
影响因子：
16
通讯作者：
Tsukiyama, Toshio
中科院分区：
文献类型：
--
作者：
Yadon, Adam N.;Singh, Badri Nath;Hampsey, Michael;Tsukiyama, Toshio
ATP依赖的染色质重塑酶是非常丰富的，并发挥关键作用，调节DNA依赖的过程。它们靶向特定位点的机制在全基因组范围内尚未得到很好的理解。在这里，我们提出的证据表明，Isw 2染色质重塑酶的一个主要的靶向机制，以特定的基因组位点是通过序列特异性转录因子（TF）依赖的招聘。出乎意料的是，Isw2以TF依赖性方式被募集到大量没有TF结合位点的基因座。使用3C测定，我们表明，Isw2可以通过Ume6和TFIIB依赖的DNA环靶向。这些结果将DNA成环确定为一种以前未知的染色质重塑酶招募机制，并定义了DNA成环的新功能。我们还提出的证据表明，Ume6依赖的DNA循环参与染色质重塑和转录抑制，揭示了染色质的三维折叠影响DNA依赖的过程的机制。
ATP-dependent chromatin remodeling enzymes are highly abundant and play pivotal roles regulating DNA-dependent processes. The mechanisms by which they are targeted to specific loci have not been well understood on a genome-wide scale. Here we present evidence that a major targeting mechanism for the Isw2 chromatin remodeling enzyme to specific genomic loci is through sequence-specific transcription factor (TF)-dependent recruitment. Unexpectedly, Isw2 is recruited in a TF-dependent fashion to a large number of loci without TF binding sites. Using the 3C assay, we show that Isw2 can be targeted by Ume6- and TFIIB-dependent DNA looping. These results identify DNA looping as a previously unknown mechanism for the recruitment of a chromatin remodeling enzyme and defines a novel function for DNA looping. We also present evidence suggesting that Ume6-dependent DNA looping is involved in chromatin remodeling and transcriptional repression, revealing a mechanism by which the three-dimensional folding of chromatin affects DNA-dependent processes.
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影响因子：
4.3
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.1003438107
发表时间：
2010-11-16
影响因子：
11.1
作者：
Erdel, Fabian;Schubert, Thomas;Rippe, Karsten
通讯作者：
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