Engineering pedicled vascularized bladder tissue for functional bladder defect repair.
Engineering pedicled vascularized bladder tissue for functional bladder defect repair.
复制标题
工程化带蒂血管化膀胱组织用于功能性膀胱缺损修复
DOI：
10.1002/btm2.10440
复制
发表时间：
2023-09
影响因子：
7.4
通讯作者：
中科院分区：
文献类型：
--
作者：
摘要一种模拟天然膀胱结构和功能特征的工程膀胱构建物是一种有前途的膀胱替代治疗选择。我们以前的研究表明，通过将平滑肌细胞（SMC）片移植到轴向包膜血管床上制成的带蒂血管化平滑肌组织具有可靠的膀胱重建潜力。在这项研究中，我们研究了颊粘膜移植物（BMG）与带蒂血管化平滑肌组织整合以生成全层带蒂血管化膀胱结构的可行性。移植到血管化平滑肌组织上的BMG表现出良好的存活，并发育成具有全层结构、适当厚度、丰富血管化和有效屏障功能的带蒂血管化膀胱结构。然后分别用带蒂囊组织（带蒂囊组）、无蒂血管化膀胱结构（无蒂结构组）和带蒂血管化膀胱结构（带蒂结构组）重建全层膀胱缺损。天狼星红（PSR）染色和免疫组织化学结果显示，带蒂结构组中纤维化程度最低，平滑肌比例最大，血管密度较高。仅在带蒂结构组中观察到连续的粘膜层。此外，形态学和功能研究显示带蒂结构组的膀胱顺应性和延展性更好。总之，这些结果表明，BMG可以很好地与血管化平滑肌组织整合，并产生带蒂的，完全血管化的，结构有序的膀胱结构，这有利于结构重建和功能恢复，并可能成为膀胱重建的替代方案。
Abstract An engineered bladder construct that mimics the structural and functional characteristics of native bladder is a promising therapeutic option for bladder substitution. We previously showed that pedicled vascularized smooth muscle tissue fabricated by grafting smooth muscle cell (SMC) sheets onto an axial capsule vascular bed had the potential for reliable bladder reconstruction. In this study, we investigated the feasibility of buccal mucosa graft (BMG) integration with the pedicled vascularized smooth muscle tissue to generate a full‐layer pedicled vascularized bladder construct. BMG transplanted onto vascularized smooth muscle tissue showed good survival and developed into a pedicled vascularized bladder construct with full‐layer structures, appropriate thickness, abundant vascularization, and effective barrier function. Then the full‐thickness bladder defects were, respectively, reconstructed by pedicled capsule tissue (pedicled capsule group), nonpedicled vascularized bladder construct (nonpedicled construct group), and pedicled vascularized bladder construct (pedicled construct group). The picrosirius red (PSR) staining and immunohistochemistry results showed minimal fibrosis, maximal smooth muscle proportion, and high vascular density in the pedicled construct group. A continuous mucosal layer was observed only in the pedicled construct group. Moreover, morphological and functional studies revealed better bladder compliance and good ductility in the pedicled construct group. Overall, these results suggested that the BMG could be well integrated with vascularized smooth muscle tissue and generated a pedicled, fully vascularized, and structurally organized bladder construct, which facilitated structural remodeling and functional recovery and could become an alternative to bladder reconstruction.
登录
查看更多内容
影响因子：
2.6
作者：
Rajasekaran, Mahadevan;Stein, Paul;Parsons, C. Lowell
通讯作者：
Parsons, C. Lowell
影响因子：
2.3
作者：
Dawson DV;Drake DR;Hill JR;Brogden KA;Fischer CL;Wertz PW
通讯作者：
Wertz PW
DOI：
10.1007/978-981-13-5895-1_5
发表时间：
2019-01-01
期刊：
SMOOTH MUSCLE SPONTANEOUS ACTIVITY: PHYSIOLOGICAL AND PATHOLOGICAL MODULATION
影响因子：
--
作者：
Fry, Christopher H.;McCloskey, Karen D.
通讯作者：
McCloskey, Karen D.
影响因子：
2.1
作者：
Lu, Mujun;Zhou, Guangdong;Cao, Yilin
通讯作者：
Cao, Yilin
影响因子：
6.6
作者：
Joseph, David B.;Borer, Joseph G.;McLorie, Gordon A.
通讯作者：
McLorie, Gordon A.