Synchronous improvement of subgenomes in allopolyploid: a case of Sclerotinia resistance improvement in Brassica napus
Synchronous improvement of subgenomes in allopolyploid: a case of Sclerotinia resistance improvement in Brassica napus
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异源多倍体亚基因组的同步改良：以甘蓝型油菜核盘菌抗性改良为例
DOI：
10.1007/s11032-018-0915-x
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发表时间：
2018
影响因子：
3.1
通讯作者：
Qian Wei
中科院分区：
文献类型：
--
作者：
Ding Yijuan;Mei Jiaqin;Wu Qinan;Xiong Zhiyong;Li Yuehua;Shao Chaoguo;Wang Lei;Qian Wei
同源基因交换的罕见机会导致在体内异源多倍体的亚基因组之间转移优良基因座的效率很低。本文以甘蓝型油菜菌核病抗性改良为例，提出了同步改良异源多倍体亚基因组的育种策略。通过鉴定甘蓝的抗源并将抗性基因座从抗性甘蓝转移到白菜中，首次对两个亲本的抗性进行了改良。甘蓝型油菜和甘蓝型油菜的抗性基因座通过重新合成甘蓝型油菜进行聚合。对4组人工合成的甘蓝型油菜进行了3年的菌核病抗性鉴定，其中包括37个具有或不具有白菜型油菜抗性基因座的品系。叶片和茎的抗性在四组间均有显著差异。携带甘蓝型油菜和甘蓝型油菜抗病基因座的人工合成甘蓝型油菜的抗病水平最高，其中一个突出品系的抗茎抗性比部分抗病的中国油菜品种中双9高2.7倍。我们的数据表明，同步改良亚基因组的策略可以有效地改良异源多倍体。
The rare chance of homoelogous exchange results in a low efficiency to transfer elite locus between subgenomes of allopolyploid in vivo. Here, we propose a breeding strategy to synchronously improve the subgenomes of allopolyploid, as a case of Sclerotinia sclerotiorum resistance improvement in Brassica napus. The resistance of both parental species was firstly improved by identifying resistance source in B. oleracea and transferring the resistance loci from resistant B. oleracea into B. rapa. The resistance loci from B. rapa and B. oleracea were then pyramided by resynthesizing B. napus. Four groups of resynthesized B. napus, comprising of 37 lines with or without resistance loci from B. rapa and/or B. oleracea, were evaluated for Sclerotinia resistance across 3 years. Significant differences were found among the four groups for both leaf and stem resistance. The group of resynthesized B. napus carrying resistance loci from B. rapa and B. oleracea exhibited the highest level of resistance, out of which one prominent line showed 2.7-fold higher stem resistance than “Zhongshuang 9,” a partial resistant Chinese rapeseed variety. Our data highlights that the strategy of synchronous improvement of subgenomes can efficiently improve allopolyploids.
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DOI：
10.1007/s00122-002-1171-2
发表时间：
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期刊：
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