Mutational analysis of SARS-CoV-2 ORF8 during six months of COVID-19 pandemic.
Mutational analysis of SARS-CoV-2 ORF8 during six months of COVID-19 pandemic.
复制标题
新冠肺炎流行半年期间SARS-CoV-2 ORF8基因突变分析
DOI：
10.1016/j.genrep.2021.101024
复制
发表时间：
2021-06
期刊：
影响因子：
1.3
通讯作者：
El Zein L
中科院分区：
文献类型：
--
作者：
Alkhansa A;Lakkis G;El Zein L
SARS-CoV-2是COVID 19的病原体，是2019年12月下旬在武汉出现的一种新的人类病原体。SARS-CoV-2是一种正义RNA病毒，具有4种结构蛋白和6种辅助蛋白，其中ORF 8基因编码的蛋白是最高变和进化最快的基因之一。因此，该基因突变的全局表征对于致病性和诊断是重要的。在6个月的大流行期间，在45,400个ORF 8核苷酸序列中发现了240个不同的非同义突变和2个缺失，其中约一半的突变对ORF 8有害，其中四分之一位于保守氨基酸。遗传多样性分析表明，两个主要区域港口L 84 S和S24 L。L 84 S是目前最主要的突变，其次是S24 L，最早出现在美国。ORF 8变体的系统发育分析显示，L 84 S的小分支更接近蝙蝠。这是第一项揭示2020年1月至6月ORF 8全球非同义突变的研究。
SARS-CoV-2, the causal agent of COVID 19, is a new human pathogen that appeared in Wuhan, late December 2019. SARS-CoV-2 is a positive sense RNA virus, having four structural and six accessory proteins including that encoded by ORF8 gene known to be one of the most hypervariable and rapidly evolving genes. Thus, global characterization of mutations in this gene is important for pathogenicity and diagnostics. 240 different nonsynonymous mutations and 2 deletions were identified in 45,400 ORF8 nucleotide sequences during six months pandemic with about half of these variants were deleterious for ORF8, and the quarter of them were located in conserved amino acids. Genetic diversity analysis showed two main regions that harbor L84S and S24L. L84S is by far the most predominant mutation, followed by S24L that appeared first in USA. Phylogenetic analysis of ORF8 variants revealed the appearance of small clades with that of L84S being closer to bats. This is the first study that revealed the global nonsynonymous mutations in ORF8 from January to June 2020.
登录
查看更多内容
影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
影响因子：
13.2
作者：
Chan, Jasper Fuk-Woo;Kok, Kin-Hang;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM
影响因子：
5.8
作者：
Choi, Yongwook;Chan, Agnes P.
通讯作者：
Chan, Agnes P.