Cell cycle-dependent alteration in NAC1 nuclear body dynamics and morphology.
Cell cycle-dependent alteration in NAC1 nuclear body dynamics and morphology.
复制标题
DOI：
10.1088/1478-3975/8/1/015005
复制
发表时间：
2011-02
期刊：
影响因子：
2
通讯作者：
Tseng Y
中科院分区：
文献类型：
--
作者：
Wu PH;Hung SH;Ren T;Shih IeM;Tseng Y
NAC 1是BTB/POZ家族的成员之一，参与维持胚胎干细胞的干细胞性，并与人类癌症的发病机制有关。在卵巢癌中，NAC 1上调与疾病侵袭性和化疗耐药性的发展相关。与其他BTB/POZ蛋白一样，NAC 1在非分裂细胞中形成离散的核体。为了研究NAC 1核体的生物学作用，我们表征了NAC 1核体在细胞周期的不同阶段的表达动态。荧光恢复后的光漂白试验表明，NAC 1迅速交换之间的核质和NAC 1核体在间期细胞。与G 0/G1期和G2期相比，S期NAC 1小体的数量显著增加，体积显著减小。在有丝分裂过程中，NAC 1核体消失，NAC 1变得弥散。NAC 1核体在有丝分裂完成后立即重新出现。这些结果表明，细胞周期依赖的调节机制控制NAC 1体形成的细胞核，并建议NAC 1体动力学与有丝分裂或胞质分裂。
NAC1, a BTB/POZ family member, has been suggested to participate in maintaining stemness of embryonic stem cells and has been implicated in the pathogenesis of human cancer. In ovarian cancer, NAC1 upregulation is associated with disease aggressiveness and with the development of chemoresistance. Like other BTB/POZ proteins, NAC1 forms discrete nuclear bodies in non-dividing cells. To investigate the biologic role of NAC1 nuclear bodies, we characterized the expression dynamics of NAC1 nuclear bodies during different phases of the cell cycle. Fluorescence recovery after photobleaching assays revealed that NAC1 was rapidly exchanged between the nucleoplasm and NAC1 nuclear bodies in interphase cells. The number of NAC1 bodies significantly increased and their size decreased in S-phase as compared to G0/G1 and G2 phases. NAC1 nuclear bodies disappeared and NAC1 became diffuse during mitosis. NAC1 nuclear bodies reappeared immediately after completion of mitosis. These results indicate that a cell cycle-dependent regulatory mechanism controls NAC1 body formation in the nucleus and suggest that NAC1 body dynamics are associated with mitosis or cytokinesis.
登录
查看更多内容
临时细胞性白血病核体与转录活性基因组区域相关。
DOI：
10.1083/jcb.200305142
发表时间：
2004-02-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Wang J;Shiels C;Sasieni P;Wu PJ;Islam SA;Freemont PS;Sheer D
通讯作者：
Sheer D
影响因子：
12.3
作者：
Stogios, Peter J;Downs, Gregory S;Jauhal, Jimmy J S;Nandra, Sukhjeen K;Prive, Gilbert G
通讯作者：
Prive, Gilbert G
影响因子：
11.2
作者：
Nakayama, Kentaro;Nakayama, Naomi;Shih, Ie-Ming
通讯作者：
Shih, Ie-Ming
影响因子：
3.8
作者：
Wu PH;Nelson N;Tseng Y
通讯作者：
Tseng Y
影响因子：
254.7
作者：
Siegel, Rebecca L.;Miller, Kimberly D.;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin