Identification of pheromone components and their binding affinity to the odorant binding protein CcapOBP83a-2 of the Mediterranean fruit fly, Ceratitis capitata.
Identification of pheromone components and their binding affinity to the odorant binding protein CcapOBP83a-2 of the Mediterranean fruit fly, Ceratitis capitata.
复制标题
DOI：
10.1016/j.ibmb.2014.02.005
复制
发表时间：
2014-05
影响因子：
3.8
通讯作者：
Zhou, J. J.
中科院分区：
文献类型：
--
作者：
Siciliano, P.;He, X. L.;Woodcock, C.;Pickett, J. A.;Field, L. M.;Birkett, M. A.;Kalinova, B.;Gomulski, L. M.;Scolari, F.;Gasperi, G.;Malacrida, A. R.;Zhou, J. J.
关键词：
地中海实蝇Ceratitis capitata（Wiedemann;双翅目：实蝇科）是一种严重的世界性农业害虫，其幼虫寄主范围非常广，超过250种不同的水果和蔬菜。嗅觉在该物种的入侵潜力中起着关键作用。不幸的是，地中海果蝇的信息素通信系统是复杂的，仍然没有很好地建立。在这项研究中，我们报告的隔离性成熟的个人在“呼叫”期间发出的化学物质和电生理反应，这些化合物引起的触角的男性和女性苍蝇。采用气相色谱-触角电位联用技术（GC-EAG），从雄性大鼠的鼻分泌物中分离鉴定了15种具有电生理活性的化合物。在该组的15个确定的化合物，11引起的反应，在触角的两性，而4引起的反应，仅在女性触角。使用C. capitata OBP，CcapOBP 83 a-2测量这些化合物加上其他研究中已知具有行为活性的4种其他化合物的结合亲和力。该OBP与果蝇OBP OS-E和OS-F具有高度同源性，OS-E和OS-F与果蝇感器相关，并与已充分研究的果蝇信息素结合蛋白LUSH共表达。这些结果提供了CcapOBP 83 a-2参与地中海果蝇气味感知的证据，以及其对地中海果蝇雄性信息素释放的五种主要化合物之一（E，E）-α-法尼烯的更广泛特异性。这代表了澄清结头瓢虫和信息素接收途径的第一步，也是进一步研究的起点，旨在创造适用于实际控制策略的新的强大引诱剂或驱避剂。利用气引法和GC-EAG法研究了地中海果蝇雄虫在求偶期产生的混合信息素。在15种化学物质中，有11种引起了两性触角的反应，4种只引起雌性触角的反应。第一种地中海果蝇气味结合蛋白CcapOBP 83 a-2已被表达和纯化（87）。CcapOBP 83 a-2对地中海果蝇信息素的一种成分（E，E）-α-法尼烯具有结合亲和力。CcapOBP 83 a-2结合其他电生理活性化合物的能力显示其广泛的结合特异性。
The Mediterranean fruit fly (or medfly), Ceratitis capitata (Wiedemann; Diptera: Tephritidae), is a serious pest of agriculture worldwide, displaying a very wide larval host range with more than 250 different species of fruit and vegetables. Olfaction plays a key role in the invasive potential of this species. Unfortunately, the pheromone communication system of the medfly is complex and still not well established. In this study, we report the isolation of chemicals emitted by sexually mature individuals during the “calling” period and the electrophysiological responses that these compounds elicit on the antennae of male and female flies. Fifteen compounds with electrophysiological activity were isolated and identified in male emissions by gas chromatography coupled to electroantennography (GC–EAG). Within the group of 15 identified compounds, 11 elicited a response in antennae of both sexes, whilst 4 elicited a response only in female antennae. The binding affinity of these compounds, plus 4 additional compounds known to be behaviourally active from other studies, was measured using C. capitata OBP, CcapOBP83a-2. This OBP has a high homology to Drosophila melanogaster OBPs OS-E and OS-F, which are associated with trichoid sensilla and co-expressed with the well-studied Drosophila pheromone binding protein LUSH. The results provide evidence of involvement of CcapOBP83a-2 in the medfly's odorant perception and its wider specificity for (E,E)-α-farnesene, one of the five major compounds in medfly male pheromone emission. This represents the first step in the clarification of the C. capitata and pheromone reception pathway, and a starting point for further studies aimed towards the creation of new powerful attractants or repellents applicable in the actual control strategies. The pheromone blend produced by medfly males during the calling period is investigated using air entrainment and GC–EAG. Out of 15 chemicals, 11 elicited antennal responses in both sexes and 4 elicited the response only in female antennae. The first medfly odorant binding protein CcapOBP83a-2 has been expressed and purified (87). CcapOBP83a-2 showed binding affinity for (E,E)-α-farnesene, one of the components of medfly pheromone. The ability of CcapOBP83a-2 to bind other electrophysiologically active compounds shows its broad binding specificity.
登录
查看更多内容
影响因子：
1.9
作者：
LEVINSON, HZ;LEVINSON, AR;SCHAFER, K
通讯作者：
SCHAFER, K
影响因子：
3.7
作者：
Gomulski LM;Dimopoulos G;Xi Z;Scolari F;Gabrieli P;Siciliano P;Clarke AR;Malacrida AR;Gasperi G
通讯作者：
Gasperi G
影响因子：
6.1
作者：
Alfaro, Cristina;Vacas, Sandra;Primo, Jaime
通讯作者：
Primo, Jaime
影响因子：
7
作者：
Hekmat-Scafe, DS;Scafe, CR;Tanouye, MA
通讯作者：
Tanouye, MA
影响因子：
7.3
作者：
JACOBSON, M;OHINATA, K;FUJIMOTO, MS
通讯作者：
FUJIMOTO, MS