Combining hot-compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptus.
Combining hot-compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptus.
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DOI：
10.1186/1754-6834-1-2
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发表时间：
2008-04-15
影响因子：
6.3
通讯作者：
Sawayama S
中科院分区：
文献类型：
--
作者：
Inoue H;Yano S;Endo T;Sakaki T;Sawayama S
木质纤维生物质，如木材，是一种有吸引力的燃料乙醇生产材料。提高木质纤维生物质中纤维素和半纤维素消化率的前处理技术对后续的酶解和乙醇发酵过程的成本有很大的影响。没有酸、碱或有机溶剂等化学物质的前处理对酶解过程的效果不如有化学物质的前处理，但它们对环境的负面影响较小。采用球磨(BM)和热压缩水(HCW)处理相结合的方法，对桉树的酶消化性能进行了研究。BM处理同时提高了葡聚糖和木聚糖的消化率，与HCW处理相比，BM处理有效地降低了酶负荷。HCW与BM联合治疗可缩短BM时间。用HCW(160℃，30min)和BM(20min)处理桉树，总糖得率约为70%，纤维素酶载量为4FPU/g底物。这一产率与HCW(200℃，30分钟)或BM(40分钟)处理的样品的产率相当，底物含量为40 FPU/g。HCW处理有利于提高球磨效率。HCW-BM组合处理可节约能源和酶负荷。
Lignocellulosic biomass such as wood is an attractive material for fuel ethanol production. Pretreatment technologies that increase the digestibility of cellulose and hemicellulose in the lignocellulosic biomass have a major influence on the cost of the subsequent enzymatic hydrolysis and ethanol fermentation processes. Pretreatments without chemicals such as acids, bases or organic solvents are less effective for an enzymatic hydrolysis process than those with chemicals, but they have a less negative effect on the environment. The enzymatic digestibility of eucalyptus was examined following a combined pretreatment without chemicals comprising a ball milling (BM) and hot-compressed water (HCW) treatment. The BM treatment simultaneously improved the digestibility of both glucan and xylan, and was effective in lowering the enzyme loading compared with the HCW treatment. The combination of HCW and BM treatment reduced the BM time. The eucalyptus treated with HCW (160°C, 30 minutes) followed by BM (20 minutes) had an approximately 70% yield of total sugar with a cellulase loading of 4 FPU/g substrate. This yield was comparable to the yields from samples treated with HCW (200°C, 30 minutes) or BM (40 minutes) hydrolyzed with 40 FPU/g substrate. The HCW treatment is useful in improving the milling efficiency. The combined HCW-BM treatment can save energy and enzyme loading.
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影响因子：
11.4
作者：
Lloyd, TA;Wyman, CE
通讯作者：
Wyman, CE
影响因子：
4.2
作者：
MOK, WSL;ANTAL, MJ
通讯作者：
ANTAL, MJ
影响因子：
11.4
作者：
Vlasenko, EY;Ding, H;Shoemaker, SP
通讯作者：
Shoemaker, SP
影响因子：
3
作者：
Chang, VS;Holtzapple, MT
通讯作者：
Holtzapple, MT
影响因子：
3.1
作者：
Kabel, MA;de Waard, P;Voragen, AGJ
通讯作者：
Voragen, AGJ