The MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset.
The MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset.
复制标题
DOI：
10.1093/nar/gkr869
复制
发表时间：
2012-02
影响因子：
14.9
通讯作者：
Becker PB
中科院分区：
文献类型：
--
作者：
Feller C;Prestel M;Hartmann H;Straub T;Söding J;Becker PB
MOF（男性缺席的第一）含有NSL（非特异性致死）复合物结合到一个子集的活性启动子在果蝇，并被认为有助于适当的基因表达。将NSL靶向特定启动子的决定簇以及复合物参与调节转录的情况目前尚不清楚。在这里，我们表明，NSL复合物主要针对活性启动子，特别是管家基因，在那里它与染色质重塑因子（nucleosome remodeling factor）和组蛋白甲基转移酶Trithorax共定位。然而，只有一个与NSL相关的管家基因的子集实际上被它激活。我们的分析表明，这些NSL激活的启动子耗尽某些绝缘子结合蛋白，并富含核心启动子基序'Ohler 5'。基于这些结果，可以预测NSL复合物是否可能调节特定的启动子。我们的结论是，监管能力的NSL复杂的是高度依赖于上下文。通过NSL复合物的激活需要由染色质调节剂和核心启动子基序的组合限定的特定启动子结构。
The MOF (males absent on the first)-containing NSL (non-specific lethal) complex binds to a subset of active promoters in Drosophila melanogaster and is thought to contribute to proper gene expression. The determinants that target NSL to specific promoters and the circumstances in which the complex engages in regulating transcription are currently unknown. Here, we show that the NSL complex primarily targets active promoters and in particular housekeeping genes, at which it colocalizes with the chromatin remodeler NURF (nucleosome remodeling factor) and the histone methyltransferase Trithorax. However, only a subset of housekeeping genes associated with NSL are actually activated by it. Our analyses reveal that these NSL-activated promoters are depleted of certain insulator binding proteins and are enriched for the core promoter motif ‘Ohler 5’. Based on these results, it is possible to predict whether the NSL complex is likely to regulate a particular promoter. We conclude that the regulatory capacity of the NSL complex is highly context-dependent. Activation by the NSL complex requires a particular promoter architecture defined by combinations of chromatin regulators and core promoter motifs.
登录
查看更多内容
影响因子：
16.8
作者：
通讯作者：
--
影响因子：
2.7
作者：
Juven-Gershon, Tamar;Kadonaga, James T.
通讯作者：
Kadonaga, James T.
DOI：
10.1038/nrg2847
发表时间：
2010-08
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.5
作者：
Kaplan T;Li XY;Sabo PJ;Thomas S;Stamatoyannopoulos JA;Biggin MD;Eisen MB
通讯作者：
Eisen MB
影响因子：
10.5
作者：
Gilfillan, GD;Straub, T;Becker, PB
通讯作者：
Becker, PB