Vagal Nerve Stimulation for Glycemic Control in a Rodent Model of Type 2 Diabetes.
Vagal Nerve Stimulation for Glycemic Control in a Rodent Model of Type 2 Diabetes.
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DOI：
10.1007/s11695-019-03901-9
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发表时间：
2019-09
期刊：
影响因子：
2.9
通讯作者：
Chen, Jiande D. Z.
中科院分区：
文献类型：
--
作者：
Yin, Jieyun;Ji, Feng;Gharibani, Payam;Chen, Jiande D. Z.
在基础和临床研究中，迷走神经刺激（VNS）已被报道可以减轻体重和改善交感病理迷走神经失衡。然而，它对血糖控制的影响尚不清楚。本研究旨在探讨不同参数VNS对大鼠血糖的影响及其可能的机制。初步采用胰高血糖素致高血糖鼠模型，优化VNS参数；然后，采用高脂饮食联合链脲佐菌素诱导的2型糖尿病啮齿动物模型验证VNS方法。VNS电极植入膈下迷走神经背侧；在胰高血糖素诱导大鼠胸部皮下植入3个电极记录心电图。(1)短脉宽0.3 ms而非3 ms的VNS在口服葡萄糖耐量试验（OGTT）中降低血糖，15 min和30 min分别降低38.4%和26.9% （P < 0.05，与假VNS相比）。(2)低频率VNS在OGTT期间血糖降低（P < 0.05，与假VNS相比），而低频率VNS在14hz和40hz时血糖降低（P < 0.05）。(3)间歇性VNS比连续性VNS更有效（P < 0.01）。(4)单侧迷走神经与双侧迷走神经无明显差异。(5) VNS增强迷走神经活动（P = 0.005）。(6) VNS的降糖作用被胰高血糖素样肽-1 （GLP-1）拮抗剂exendin-4阻断。5hz VNS通过增强迷走神经传出活动和GLP-1的释放来降低糖尿病大鼠的血糖。
Vagal nerve stimulation (VNS) has been reported to reduce body weight and improve sympathovagal imbalance in both basic and clinical studies. Its effects on glycemic control were however unclear. The aims of this study were to investigate the effects of VNS with various parameters on blood glucose and its possible mechanisms in rats. A hyperglycemic rodent model induced by glucagon was used initially to optimize the VNS parameters; then, a type 2 diabetic rodent model induced by high-fat diet combined with streptozotocin was used to validate the VNS method. The VNS electrodes were implanted at the dorsal subdiaphragmatic vagus; three subcutaneous electrodes were implanted at the chest area for recording electrocardiogram in rats induced by glucagon. (1) VNS with short pulse width of 0.3 ms but not 3 ms reduced blood glucose during an oral glucose tolerance test (OGTT), with a 38.4% reduction at 15 min and 26.9% at 30 min (P < 0.05, vs. sham-VNS respectively). (2) VNS at low frequency of 5 Hz but not 14 Hz or 40 Hz reduced blood glucose during the OGTT (P < 0.05, vs. sham-VNS). (3) Intermittent VNS was more potent than continuous VNS (P < 0.01). (4) No difference was found between unilateral VNS and bilateral VNS. (5) VNS enhanced vagal activity (P = 0.005). (6) The hypoglycemic effect of VNS was blocked by glucagon-like peptide-1 (GLP-1) antagonist exendin-4. VNS at 5 Hz reduces blood glucose in diabetic rats by enhancing vagal efferent activity and the release of GLP-1.
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影响因子：
2.9
作者：
Morton JM;Shah SN;Wolfe BM;Apovian CM;Miller CJ;Tweden KS;Billington CJ;Shikora SA
通讯作者：
Shikora SA
影响因子：
3.7
作者：
Banni S;Carta G;Murru E;Cordeddu L;Giordano E;Marrosu F;Puligheddu M;Floris G;Asuni GP;Cappai AL;Deriu S;Follesa P
通讯作者：
Follesa P
影响因子：
7.7
作者：
Krieger, Jean-Philippe;Arnold, Myrtha;Lee, Shin J.
通讯作者：
Lee, Shin J.
影响因子：
4.8
作者：
AHREN, B;TABORSKY, GJ
通讯作者：
TABORSKY, GJ
影响因子：
10.6
作者：
George, MS;Sackeim, HA;Ballenger, JC
通讯作者：
Ballenger, JC