Campylobacter jejuni transcriptome changes during loss of culturability in water.
Campylobacter jejuni transcriptome changes during loss of culturability in water.
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DOI：
10.1371/journal.pone.0188936
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
ENIGMA Consortium
中科院分区：
文献类型：
--
作者：
Bronowski C;Mustafa K;Goodhead I;James CE;Nelson C;Lucaci A;Wigley P;Humphrey TJ;Williams NJ;Winstanley C;ENIGMA Consortium
水是弯曲杆菌的潜在宿主，弯曲杆菌是人类细菌性胃肠炎的主要原因。然而，很少有人了解的机制，潜在的变化，在生存特性之间的不同菌株的C。自然环境中的空肠，包括水。我们鉴定了三种空肠弯曲杆菌菌株，其在两种不同温度（4 ° C和25 ° C）下悬浮于自来水中后保持可培养性的能力表现出可变性。其中C.空肠M1表现出最快的可培养性损失，同时保持活力。使用RNAseq转录组学，我们表征了C.通过分析引入水中后立即回收的细菌悬浮液（时间0），以及来自两个采样时间/温度组合的细菌悬浮液，即（i）在25 ° C下24小时后，和（ii）在4 ° C下72小时后，对空肠M1基因表达的响应，其中可培养性明显丧失。将转录数据与培养生长的对照进行比较。从水中回收的三个种群之间共享一些基因表达特征，上调的基因多于下调的基因。在时间0样品中鉴定了许多上调基因，而大多数下调基因发生在25 ° C（24 h）样品中。在与耐氧性、饥饿和渗透胁迫相关的基因中发现了表达的变化。然而，我们也发现鞭毛组装基因的上调，伴随着参与趋化性的基因下调。我们的数据还表明，开关分泌通过秒系统，通过tat系统，和群体感应基因luxS可能与菌株M1在水中的生存。基因表达的变化也发生在辅助基因组区域。我们的数据表明，尽管失去了可培养性，C。空肠M1保持活力，并通过基因表达的特定变化进行适应。
Water serves as a potential reservoir for Campylobacter, the leading cause of bacterial gastroenteritis in humans. However, little is understood about the mechanisms underlying variations in survival characteristics between different strains of C. jejuni in natural environments, including water. We identified three Campylobacter jejuni strains that exhibited variability in their ability to retain culturability after suspension in tap water at two different temperatures (4°C and 25°C). Of the three, strains C. jejuni M1 exhibited the most rapid loss of culturability whilst retaining viability. Using RNAseq transcriptomics, we characterised C. jejuni M1 gene expression in response to suspension in water by analyzing bacterial suspensions recovered immediately after introduction into water (Time 0), and from two sampling time/temperature combinations where considerable loss of culturability was evident, namely (i) after 24 h at 25°C, and (ii) after 72 h at 4°C. Transcript data were compared with a culture-grown control. Some gene expression characteristics were shared amongst the three populations recovered from water, with more genes being up-regulated than down. Many of the up-regulated genes were identified in the Time 0 sample, whereas the majority of down-regulated genes occurred in the 25°C (24 h) sample. Variations in expression were found amongst genes associated with oxygen tolerance, starvation and osmotic stress. However, we also found upregulation of flagellar assembly genes, accompanied by down-regulation of genes involved in chemotaxis. Our data also suggested a switch from secretion via the sec system to via the tat system, and that the quorum sensing gene luxS may be implicated in the survival of strain M1 in water. Variations in gene expression also occurred in accessory genome regions. Our data suggest that despite the loss of culturability, C. jejuni M1 remains viable and adapts via specific changes in gene expression.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3.7
作者：
Drozd M;Gangaiah D;Liu Z;Rajashekara G
通讯作者：
Rajashekara G
影响因子：
4.2
作者：
Bui XT;Qvortrup K;Wolff A;Bang DD;Creuzenet C
通讯作者：
Creuzenet C
影响因子：
4.4
作者：
Gangaiah, Dharanesh;Kassem, Issmat I.;Rajashekara, Gireesh
通讯作者：
Rajashekara, Gireesh
影响因子：
3.7
作者：
Friis C;Wassenaar TM;Javed MA;Snipen L;Lagesen K;Hallin PF;Newell DG;Toszeghy M;Ridley A;Manning G;Ussery DW
通讯作者：
Ussery DW