Newly emerged porcine enteric alphacoronavirus in southern China: Identification, origin and evolutionary history analysis.
Newly emerged porcine enteric alphacoronavirus in southern China: Identification, origin and evolutionary history analysis.
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DOI：
10.1016/j.meegid.2018.04.031
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Fu X;Fang B;Liu Y;Cai M;Jun J;Ma J;Bu D;Wang L;Zhou P;Wang H;Zhang G
冠状病毒的宿主范围广泛，可在不同动物中引起多种严重程度不同的疾病。已发现几种肠道冠状病毒与猪腹泻有关，并造成了巨大的经济损失。本研究从中国南方腹泻乳猪中鉴定出一种新出现的猪肠道甲型冠状病毒（PEAV）PEAV-GD-CH/2017，并获得了PEAV全长基因组序列进行系统分析。新的PEAV序列与bat-HKU2的序列最为一致，并全面比较了它们之间的差异，特别是S蛋白的一致特征，显示其与β冠状病毒有密切的关系，并且可能代表未被识别的β冠状病毒。此外，针对PEAV的起源进行了贝叶斯分析，估计PEAV与bat-HKU2之间的分歧时间为1926年，这表明PEAV并不是新出现的，自这种冠状病毒从蝙蝠到猪的种间传播以来，PEAV可能已经在猪群中传播了几十年。在我们的分析中，冠状病毒的进化速率估计为每年每个位点 RdRp 基因发生 1.93×10−4 次替换。对于PEAV的起源，我们怀疑它是bat-HKU2从蝙蝠到猪的种间传播的结果。我们的研究结果提供了有关新型 PEAV 的统一特征、起源和进化的宝贵信息，这将有助于对这种新出现的病原体的进一步研究。对来自腹泻哺乳仔猪的 PEAV 菌株进行鉴定和测序 PEAV 的 S 蛋白可能是由未被识别的 β-CoV 重组而来。新的 PEAV 大约在 1926 年基于贝叶斯分析出现。 PEAV 起源于 bat-HKU2 从蝙蝠到猪的种间传播
Coronaviruses have a wide host range and can cause a variety of diseases with varying severity in different animals. Several enteric coronaviruses have been identified that are associated with diarrhea in swine and that have caused substantial economic losses. In this study, a newly emerged porcine enteric alphacoronavirus (PEAV), PEAV-GD-CH/2017, was identified from suckling piglets with diarrhea in southern China, and a full-length genome sequence of PEAV was obtained for systematic analysis. The novel PEAV sequence was most identical to that of bat-HKU2, and the differences between them were comprehensively compared, especially the uniform features of the S protein, which was shown to have a close relationship with betacoronaviruses and to perhaps represent unrecognized betacoronaviruses. In addition, Bayesian analysis was conducted to address the origin of PEAV, and the divergence time between PEAV and bat-HKU2 was estimated at 1926, which indicates that PEAV is not newly emerged and may have circulated in swine herds for several decades since the interspecies transmission of this coronavirus from bat to swine. The evolutionary rate of coronaviruses was estimated to be 1.93 × 10−4 substitutions per site per year for the RdRp gene in our analysis. For the origin of PEAV, we suspect that it is the result of the interspecies transmission of bat-HKU2 from bat to swine. Our results provide valuable information about the uniform features, origin and evolution of the novel PEAV, which will facilitate further investigations of this newly emerged pathogen. Identify and sequence a PEAV strain from suckling piglets with diarrhea The S protein of PEAV may recombination from unrecognized beta-CoV. The novel PEAV was emerged approximately at 1926 based on Bayesian analysis. PEAV origin from the interspecies transmission of bat-HKU2 from bat to swine
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