Detection and genetic analysis of porcine hemagglutinating encephalomyelitis virus in South Korea.

Detection and genetic analysis of porcine hemagglutinating encephalomyelitis virus in South Korea.
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韩国猪血凝性脑脊髓炎病毒的检测和遗传分析。

DOI:
10.1007/s11262-010-0551-y
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发表时间:
2011-03
期刊:
影响因子:
1.6
通讯作者:
Park BK
Park BK
中科院分区:
医学4区
文献类型:
--
作者:
Rho S;Moon HJ;Park SJ;Kim HK;Keum HO;Han JY;Van Nguyen G;Park BK

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猪血凝性脑脊髓炎病毒(PHEV)引起呕吐和消耗性疾病(VWD)或脑脊髓炎,并且主要影响3周龄以下的猪。在这项研究中,我们检测PHEV从临床病猪在传统的养猪场在韩国。2009年11月至2010年3月,采用巢式RT-PCR方法检测了来自91个猪场的239份猪组织样品。在239份样品中,来自17个农场的22份样品对PHEV呈阳性。哺乳猪、断奶猪、生长猪和育肥猪的检出率分别为14.3%(12/84)、6.5%(7/107)、7%(3/43)和0%(0/5)。症状为神经系统、呼吸系统、肠道体征(腹泻)或鼻出血。所有猪均合并感染其他病毒和细菌,这可能导致受影响猪的年龄变化和临床体征。系统进化分析表明,PHEV阳性株与参考株聚在同一群。这些发现意味着PHEV仅存在一个基因组,而与猪龄、临床体征和地理位置无关。
Porcine hemagglutinating encephalomyelitis virus (PHEV) causes vomiting and wasting disease (VWD) or encephalomyelitis, and primarily affects pigs under 3 weeks of age. In this study, we detected PHEV from clinically ill pigs in conventional pig farms in South Korea. From November 2009 to March 2010, a total of 239 pig tissue samples from 91 farms were tested by nested RT-PCR. Among 239 samples, 22 samples from 17 farms were positive for PHEV. The detection rate of suckling pigs, weaning pigs, growers and finishers were 14.3% (12/84), 6.5% (7/107), 7% (3/43), and 0% (0/5), respectively. Symptoms were neurological, respiratory, enteric sign (diarrhea), or nasal bleeding. All pigs were co-infected with other viruses and bacteria and this might have resulted in age variation and clinical signs in the affected pigs. Phylogenetic analysis showed that the PHEV-positive samples and PHEV reference strains were clustered in the same group. These findings imply the presence of only one genogroup of PHEV, regardless of porcine age, clinical signs, and geographical location.
猪腹泻病毒chinju99的核蛋白基因基因的克隆和序列分析。
DOI: 10.1023/a:1023447732567
发表时间: 2003
期刊: Virus genes
影响因子: 1.6
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通讯作者: Yeo SG
DOI: 10.1128/jvi.02675-05
发表时间: 2006-07-01
影响因子: 5.4
作者:
Vijgen, Leen;Keyaerts, Els;Van Ranst, Marc
通讯作者: Van Ranst, Marc
DOI: 10.1017/s0950268899002332
发表时间: 1999-06-01
影响因子: 4.2
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DOI: 10.1637/7186-033004r
发表时间: 2004-09-01
期刊: AVIAN DISEASES
影响因子: 1.4
作者:
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通讯作者: Wang, CH
DOI: 10.1099/0022-1317-83-10-2411
发表时间: 2002-10-01
影响因子: 3.8
作者:
Sasseville, AMJ;Boutin, M;Dea, S
通讯作者: Dea, S