Identification and High-Resolution Imaging of α-Tocopherol from Human Cells to Whole Animals by TOF-SIMS Tandem Mass Spectrometry.
Identification and High-Resolution Imaging of α-Tocopherol from Human Cells to Whole Animals by TOF-SIMS Tandem Mass Spectrometry.
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DOI：
10.1007/s13361-018-1979-x
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发表时间：
2018-08
影响因子：
3.2
通讯作者：
Heeren RMA
中科院分区：
文献类型：
--
作者：
Bruinen AL;Fisher GL;Balez R;van der Sar AM;Ooi L;Heeren RMA
采用一种独特的方法来识别不同生物样本中的生物分子成分，同时保留高速 2D 和 3D 分子成像的能力，用于研究细胞对氧化应激的反应。所采用的方法能够通过飞行时间二次离子质谱（TOF-SIMS（MS1））成像与串联质谱（MS2）成像并行观察细胞结构中抗氧化剂α-生育酚和其他分子的分布，同时收集。所述方法用于检查由人类诱导多能干细胞（iPSC）分化的神经元细胞形成的网络，这是体外研究人类神经元的模型。利用 3D TOF-SIMS 串联 MS 成像分析在不同细胞层中原位鉴定抗氧化剂 α-生育酚。由于氧化应激在介导炎症中也发挥着重要作用，因此该研究扩展到了受海洋分枝杆菌感染的斑马鱼（一种结核病模型生物）的全身组织切片。 TOF-SIMS 串联 MS 成像结果显示，针对病原体的反应中 α-生育酚的存在增加。 ᅟ 本文的在线版本 (10.1007/s13361-018-1979-x) 包含补充材料，可供授权用户使用。
A unique method for identification of biomolecular components in different biological specimens, while preserving the capability for high speed 2D and 3D molecular imaging, is employed to investigate cellular response to oxidative stress. The employed method enables observing the distribution of the antioxidant α-tocopherol and other molecules in cellular structures via time-of-flight secondary ion mass spectrometry (TOF-SIMS (MS1)) imaging in parallel with tandem mass spectrometry (MS2) imaging, collected simultaneously. The described method is employed to examine a network formed by neuronal cells differentiated from human induced pluripotent stem cells (iPSCs), a model for investigating human neurons in vitro. The antioxidant α-tocopherol is identified in situ within different cellular layers utilizing a 3D TOF-SIMS tandem MS imaging analysis. As oxidative stress also plays an important role in mediating inflammation, the study was expanded to whole body tissue sections of M. marinum-infected zebrafish, a model organism for tuberculosis. The TOF-SIMS tandem MS imaging results reveal an increased presence of α-tocopherol in response to the pathogen. ᅟ The online version of this article (10.1007/s13361-018-1979-x) contains supplementary material, which is available to authorized users.
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