Fluorescence lifetime spectroscopy for guided therapy of brain tumors.
Fluorescence lifetime spectroscopy for guided therapy of brain tumors.
复制标题
荧光寿命光谱法，用于脑肿瘤的指导治疗。
DOI：
10.1016/j.neuroimage.2010.11.001
复制
发表时间：
2011-01
期刊：
影响因子：
5.7
通讯作者：
Marcu L
中科院分区：
文献类型：
--
作者：
Butte PV;Mamelak AN;Nuno M;Bannykh SI;Black KL;Marcu L
本研究评估了时间分辨激光诱导荧光光谱（TR-LIFS）作为术中工具描绘脑肿瘤与正常大脑的潜力。本研究纳入了 42 名接受神经胶质瘤（WHO I-IV 级）手术的患者。 TR-LIFS 原型设备（门控检测、快速数字化仪）用于使用脉冲 N2 激光（337 nm 激发，0.7 ns 脉冲宽度）诱导体内荧光，并记录时间分辨光谱（360 – 550 nm 范围，10 nm 间隔）。 TR-LIFS 测量的位点通过常规组织病理学（H&E 染色）进行验证。从 TR-LIFS 数据导出的参数包括强度值和时间分辨强度衰减特征（平均荧光寿命和拉盖尔系数值），用于组织表征和分类。对肿瘤和正常大脑的 71 个区域进行了分析。有几个参数可以区分不同的组织类型。例如，与 460 nm (τ460=1.16±0.08 ns) 相比，正常皮质 (N=35) 和正常白质 (N=12) 在 390 nm (τ390=2.12±0.10 ns) 处表现出更持久的荧光发射。高级别神经胶质瘤（III 级和 IV 级）样本 (N=17) 在 460 nm 处表现出发射峰，在 390 nm 处变化较大，而低级别神经胶质瘤（I 级和 II 级）样本 (N=7) 在 460 nm 处表现出荧光发射峰。线性判别算法可以对低级别神经胶质瘤进行分类，灵敏度为 100%，特异性为 98%。高级别神经胶质瘤表现出高度的异质性，从而将这些肿瘤的辨别准确度降低至 47% 的敏感性和 94% 的特异性。目前的研究结果表明，TR-LIFS 具有在术中诊断脑肿瘤的潜力，并为帮助神经外科医生-神经病理学家团队在手术期间快速区分肿瘤和正常大脑提供了宝贵的工具。
This study evaluates the potential of time-resolved laser induced fluorescence spectroscopy (TR-LIFS) as intra-operative tool for the delineation of brain tumor from normal brain. Forty two patients undergoing glioma (WHO grade I-IV) surgery were enrolled in this study. A TR-LIFS prototype apparatus (gated detection, fast digitizer) was used to induce in-vivo fluorescence using a pulsed N2 laser (337 nm excitation, 0.7 ns pulse width) and to record the time-resolved spectrum (360 – 550 nm range, 10 nm interval). The sites of TR-LIFS measurement were validated by conventional histopathology (H&E staining). Parameters derived from the TR-LIFS data including intensity values and time-resolved intensity decay features (average fluorescence lifetime and Laguerre coefficients values) were used for tissue characterization and classification. 71 areas of tumor and normal brain were analyzed. Several parameters allowed for the differentiation of distinct tissue types. For example, normal cortex (N=35) and normal white matter (N=12) exhibit a longer-lasting fluorescence emission at 390 nm (τ390=2.12±0.10 ns) when compared with 460 nm (τ460=1.16±0.08 ns). High grade glioma (grades III and IV) samples (N=17) demonstrate emission peaks at 460 nm, with large variation at 390 nm while low grade glioma (I and II) samples (N=7) demonstrated a peak fluorescence emission at 460 nm. A linear discriminant algorithm allowed for the classification of low-grade gliomas with 100% sensitivity and 98% specificity. High-grade glioma demonstrated a high degree of heterogeneity thus reducing the discrimination accuracy of these tumors to 47% sensitivity and 94% specificity. Current findings demonstrate that TR-LIFS holds the potential to diagnose brain tumors intra-operatively and to provide a valuable tool for aiding the neurosurgeon–neuropathologist team in to rapidly distinguish between tumor and normal brain during surgery.
登录
查看更多内容
影响因子：
12.7
作者：
Hartmann, Christian;Meyer, Jochen;von Deimling, Andreas
通讯作者：
von Deimling, Andreas
DOI：
10.1016/1011-1344(90)85015-o
发表时间：
1990-03-01
影响因子：
5.4
作者：
ANDERSSONENGELS, S;JOHANSSON, J;SVANBERG, S
通讯作者：
SVANBERG, S
影响因子：
3.5
作者：
Butte, Pramod V.;Fang, Qiyin;Marcu, Laura
通讯作者：
Marcu, Laura
影响因子：
2
作者：
BARON, AE
通讯作者：
BARON, AE
影响因子：
25
作者：
Kriegeskorte, Nikolaus;Simmons, W. Kyle;Bellgowan, Patrick S. F.;Baker, Chris I.
通讯作者：
Baker, Chris I.