A Rice R2R3-Type MYB Transcription Factor OsFLP Positively Regulates Drought Stress Response via OsNAC.
A Rice R2R3-Type MYB Transcription Factor OsFLP Positively Regulates Drought Stress Response via OsNAC.
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DOI：
10.3390/ijms23115873
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发表时间：
2022-05-24
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
非生物胁迫对世界范围内的植物生长和作物产量产生不利影响。R2R3-MYB转录因子在植物逆境反应中起着至关重要的作用。在拟南芥中，四个LIPs(FLP，MYB124)及其类似的MYB88功能冗余地调节保卫母细胞的对称分裂和非生物胁迫反应。在这里，OsFLP被确定为R2R3-MYB转录激活因子，并定位于细胞核。OsFLP受到干旱、盐胁迫和脱落酸(ABA)的短暂诱导。OsFLP的过表达增强了植株对干旱和盐分胁迫的耐受性。与野生型植物相比，OsFLP-OE植株的气孔密度没有变化，而气孔关闭对ABA处理敏感。相比之下，OsFLP-RNAi植株气孔异常，对干旱敏感。此外，在OsFLP-RNAi植株中，与气孔关闭相关的基因DST和过氧化物酶24前体的转录受到抑制，这两个基因被鉴定为OsNAC1的下游基因。酵母单杂交实验表明，OsFLP能特异性结合并正向调节OsNAC1和OsNAC6。同时，OsFLP-OE植物中的胁迫反应基因，如OsLEA3和OsDREB2A表达上调。这些结果表明，OsFLP积极参与干旱胁迫，主要是通过调控OsNAC1和OsNAC6的转录。
Abiotic stresses adversely affect plant growth and the yield of crops worldwide. R2R3-MYB transcriptional factors have been found to be vital for plants to confer stress response. In Arabidopsis, FOUR LIPS (FLP, MYB124) and its paralogous MYB88 function redundantly regulated the symmetric division of guard mother cells (GMCs) and abiotic stress response. Here, OsFLP was identified as an R2R3-MYB transcriptional activator and localized in the nucleus. OsFLP was transiently induced by drought, salt stress and abscisic acid (ABA). Overexpression of OsFLP showed enhanced tolerance to drought and salt stresses. The stomatal density in OsFLP-OE plants was not changed, whereas the stomatal closure was sensitive to ABA treatment compared to wild-type plants. In contrast, OsFLP-RNAi plants had abnormal stomata and were sensitive to drought. Moreover, the transcripts of stomatal closure-related genes DST and peroxidase 24 precursor, which are identified as downstream of OsNAC1, were inhibited in OsFLP-RNAi plants. The yeast-one-hybrid assay indicated that OsFLP can specifically bind and positively regulate OsNAC1 and OsNAC6. Meanwhile, stress response genes, such as OsLEA3 and OsDREB2A, were up-regulated in OsFLP-OE plants. These findings suggested that OsFLP positively participates in drought stress, mainly through regulating regulators’ transcripts of OsNAC1 and OsNAC6.
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DOI：
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发表时间：
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期刊：
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影响因子：
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发表时间：
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期刊：
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影响因子：
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作者：
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影响因子：
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