Senescent WI-38 fibroblasts overexpress LPC-1, a putative transmembrane shock protein.
Senescent WI-38 fibroblasts overexpress LPC-1, a putative transmembrane shock protein.
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衰老的 WI-38 成纤维细胞过度表达 LPC-1，一种假定的跨膜休克蛋白。
DOI：
10.1006/excr.1998.4009
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发表时间：
1998
影响因子：
3.7
通讯作者：
Cristofalo,VJ
中科院分区：
文献类型：
--
作者：
Pignolo,RJ;Rotenberg,MO;Horton,JH;Cristofalo,VJ
我们最近报道分离了一些在非增殖性早期(年轻)和晚期(衰老)群体倍增水平(PDL)WI-38人胎肺成纤维细胞样细胞之间差异表达的基因的cDNA。我们现在证明，其中一个分离株，LPC-1(晚期PDL cDNA-1)，来自大约2.9kb的mRNA物种，在饥饿、融合、衰老的年轻WI-38细胞中，其在血清中的表达水平比类似处理的年轻细胞高2到5倍。核苷酸序列分析证实其与编码粗面内质网标记物(P63)和相关的猪肝心源性休克蛋白的cDNA同源性。我们发现LPC-1在早期PDL WI-38细胞中的表达受到严格的细胞周期调控，其表达在G0培养的血清刺激后9-12小时达到高峰。在早期PDL细胞中，LPC-1转录本在其峰值表达之前和之后的稳定水平很低，反映了去除血清后G0的基础水平。然而，晚期PDL细胞似乎已经失去了早期PDL细胞的这种紧密的细胞周期调节，并在血清刺激后不适当地表达高水平的转录本。Western分析检测到约63 kDa的蛋白，免疫组织化学检测到衰老的成纤维细胞胞浆内有强烈的染色。相关的基因组序列在所有被检查的哺乳动物物种中都发现，在鸡中也是如此。这些发现与衰老的WI-38细胞表现出与静止(G0)年轻细胞根本不同的生长停滞状态的假设一致。
We have recently reported the isolation of cDNAs for a number of genes that are differentially expressed between nonproliferating early (young) and late (senescent) population doubling level (PDL) WI-38 human, fetal lung-derived, fibroblast-like cells. We now demonstrate that one of these isolates, LPC-1 (Late PDL cDNA-1), derives from an approximately 2.9-kb mRNA species that is expressed at a two- to fivefold higher level in serum-starved, confluent, senescent versus similarly treated young WI-38 cells. Nucleotide sequence analysis of this cDNA confirms its identity with that of a cDNA encoding a marker (p63) for the rough endoplasmic recticulum and a related swine hepatic cardiogenic shock protein. We show that LPC-1 expression in early PDL WI-38 cells is strictly cell cycle-regulated and its expression peaks 9–12 h after serum stimulation of G0cultures. The steady state levels of LPC-1 transcript in early PDL cells preceeding and following its peak expression are low, reflecting basal levels seen in G0upon removal of serum. Late PDL cells, however, seem to have lost this tight cell cycle regulation seen in early PDL cells and inappropriately express high levels of the transcript after serum stimulation. Specific antiserum detects a protein of approximately 63 kDa by Western analysis and elicits intense cytoplasmic staining of senescent fibroblasts by immunohistochemistry. Related genomic sequences are found in all mammalian species examined as well as in the chicken. These findings are consistent with the hypothesis that senescent WI-38 cells exhibit a state of growth arrest fundamentally distinct from that of quiescent (G0) young cells.
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影响因子：
3.8
作者：
Buchman,TG;Cabin,DE;Vickers,S;Deutschman,CS;Delgado,E;Sussman,MM;Bulkley,GB
通讯作者：
Bulkley,GB
影响因子：
4
作者：
A. Schweizer;J. Rohrer;J. Slot;H. Geuze;S. Kornfeld
通讯作者：
A. Schweizer;J. Rohrer;J. Slot;H. Geuze;S. Kornfeld
细胞培养的进展
DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
K. Maramorosch
通讯作者：
K. Maramorosch
培养的人二倍体细胞的细胞周期特征：衰老和氢化可的松的影响
影响因子：
5.6
作者：
G. Grove;V. Cristofalo
通讯作者：
V. Cristofalo
影响因子：
4
作者：
A. Schweizer;J. Rohrer;P. Jenö;A. DeMaio;T. Buchman;H. Hauri
通讯作者：
A. Schweizer;J. Rohrer;P. Jenö;A. DeMaio;T. Buchman;H. Hauri