Possible application of Raman microspectroscopy to verify the interstitial cystitis diagnosis after potassium sensitivity test: phenylalanine or tryptophan as a biomarker.
Possible application of Raman microspectroscopy to verify the interstitial cystitis diagnosis after potassium sensitivity test: phenylalanine or tryptophan as a biomarker.
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DOI：
10.1155/2007/705630
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Lin SY
中科院分区：
文献类型：
--
作者：
Hsieh TF;Yu KJ;Lin SY
对于间质性膀胱炎（IC）的临床诊断缺乏一种世界范围内的标准技术。拉曼光谱具有较高的特异性和灵敏度，作为一种无需特殊样品制备的无损分析技术已被广泛应用。在这项初步研究中，可能使用拉曼显微光谱作为IC诊断工具进行了尝试。通过临床特征、病史、尿动力学评价和钾敏感试验（PST）对22例受试者进行筛选。使用共聚焦拉曼显微光谱法直接测定PST后所有参与者的冻干水样，以搜索生物标志物。根据上述筛选，将有或无IC症状的参与者分为对照组和临床组。临床组按PST进一步分为轻度和重度亚组。轻、重度亚组尿痛、尿急症状差异有统计学意义（p < 0.05）。与对照组相比，IC临床组患者尿频明显增加，但膀胱容量、最大膀胱容量和最大排尿流量明显减少（p < 0.05）。通过拉曼显微光谱测定，在对照组和轻度亚组冻干水样中分别检测到1003和1005 cm−1附近的苯基丙氨酸条带，而在重度亚组冻干水样中检测到色氨酸在1010 cm−1附近的条带。这项初步研究的结果首次提出了拉曼显微光谱的可能应用，以强有力地证明PST的结果用于IC诊断。苯丙氨酸或色氨酸可作为PST后IC诊断的生物标志物。特别是，色氨酸的出现可用于判别IC症状的严重程度。
There is lack of a worldwide standard technique for clinical diagnosis of interstitial cystitis (IC). Raman spectroscopy with higher specificity and sensitivity has been extensively used to act as a non-destructive analytical technique without special sample preparation. In this preliminary study, possible use of Raman microspectroscopy as an IC diagnostic tool was attempted. Twenty-two participants were screened by clinical features, history, urodynamic evaluations and potassium sensitivity test (PST). The freeze-dried water samples voided from all the participants after PST were directly determined by using a confocal Raman microspectroscopy to search the biomarker. Participants with or without IC symptom were separated into control and clinical groups, according to the above screening. The participants in the clinical group were further divided into mild and severe subgroups by PST. The symptom of urinary pain and urgency was significant difference between the mild and severe subgroups (p < 0.05). A significant increase in urinary frequency but a marked reduction in bladder capacity, maximum cystometric capacity and maximum voiding flow rate were obtained for clinical group of IC participants, as compared with the result of control group (p < 0.05). By using Raman microspectroscopic determination, the band near 1003 or 1005 cm−1 assigned to phenylalanine was respectively detected from the freeze-dried water sample of control group or mild subgroup, but the band at 1010 cm−1 due to tryptophan was found in the freeze-dried water sample of severe subgroup. The result of this preliminary study first suggests a possible application of Raman microspectroscopy to strongly certify the results of PST for IC diagnosis. Phenylalanine or tryptophan might be acted as a biomarker to assist the diagnosis of IC after PST. Particularly, the appearance of tryptophan might be used to discriminate the severity of IC symptom.
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影响因子：
2.1
作者：
STEINKOHL, WB;LEACH, GE
通讯作者：
LEACH, GE
影响因子：
7.2
作者：
Parsons, CL;Bullen, M;Willems, JJ
通讯作者：
Willems, JJ
DOI：
10.1097/00043798-200210000-00005
发表时间：
2002-10-01
期刊：
JOURNAL OF CARDIOVASCULAR RISK
影响因子：
--
作者：
van de Poll, SWE;Römer, TJ;van der Laarse, A
通讯作者：
van der Laarse, A
影响因子：
2.1
作者：
Parsons, CL;Zupkas, P;Parsons, JK
通讯作者：
Parsons, JK
影响因子：
2
作者：
Chen, KH;Cheng, WT;Lin, SY
通讯作者：
Lin, SY