Femtosecond laser cutting of human corneas for the subbasal nerve plexus evaluation
Femtosecond laser cutting of human corneas for the subbasal nerve plexus evaluation
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飞秒激光切割人角膜用于基底下神经丛评估
DOI：
10.1111/jmi.12457
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发表时间：
2017
影响因子：
2
通讯作者：
Guthoff RF
中科院分区：
文献类型：
--
作者：
Kowtharapu BS;Marfurt C;Hovakimyan M;Will F;Richter H;Wree A;Stachs O;Guthoff RF
评估角膜基底下神经丛的各种形态参数是记录健康和疾病角膜神经支配结构和功能完整性的有价值的方法。本研究的目的是建立一种快速、可靠和可重复的方法，利用飞秒激光切割角膜组织切片来可视化人类角膜收缩压。用飞秒激光显微切片机tisesurgeon固定固定健康角膜扣，在大约15分钟内获得平行于眼表的角膜上皮和前基质的厚组织切片。使用tissuergeon将近红外飞秒激光聚焦到距角膜前表面约70-90 μm处，诱导材料分离。获得的角膜切片按照标准的免疫组织化学程序用抗神经元β - III微管蛋白抗体进行染色，以显示角膜神经。包含上皮和约20-30 μm前间质的切片显示了良好的收缩压，下层间质神经的光学干扰最小。总之，本研究的结果表明，在获得平行于眼表的高质量前角膜厚切片方面，飞秒激光切割人类角膜比标准组织制备方法更快、更容易和更可靠。
Assessment of various morphological parameters of the corneal subbasal nerve plexus is a valuable method of documenting the structural and presumably functional integrity of the corneal innervation in health and disease. The aim of this work is to establish a rapid, reliable and reproducible method for visualization of the human corneal SBP using femtosecond laser cut corneal tissue sections. Trephined healthy corneal buttons were fixed and processed using TissueSurgeon—a femtosecond laser based microtome, to obtain thick tissue sections of the corneal epithelium and anterior stroma cut parallel to the ocular surface within approximately 15 min. A near infrared femtosecond laser was focused on to the cornea approximately 70–90 μm from the anterior surface to induce material separation using TissueSurgeon. The obtained corneal sections were stained following standard immunohistochemical procedures with anti‐neuronal β‐III tubulin antibody for visualization of the corneal nerves. Sections that contained the epithelium and approximately 20–30 μm of anterior stroma yielded excellent visualisation of the SBP with minimal optical interference from underlying stromal nerves. In conclusion, the results of this study have demonstrated that femtosecond laser cutting of the human cornea offers greater speed, ease and reliability than standard tissue preparation methods for obtaining high quality thick sections of the anterior cornea cut parallel to the ocular surface.
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影响因子：
4.1
作者：
Al-Aqaba, Mouhamed A.;Fares, Usama;Dua, Harminder S.
通讯作者：
Dua, Harminder S.
DOI：
10.5962/bhl.title.48637
发表时间：
--
期刊：
--
影响因子：
--
作者：
Ramón y Cajal;Santiago
通讯作者：
Ramón y Cajal;Santiago
影响因子：
2
作者：
Winter, Karsten;Scheibe, Patrick;Stachs, Oliver
通讯作者：
Stachs, Oliver
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
H. Hoyer
通讯作者：
H. Hoyer
布西拉明对激光诱导大鼠脉络膜新生血管的抑制作用
影响因子：
2
作者：
T. Hikichi;F. Mori;Mai Sasaki;A. Takamiya;Masao Nakamura;N. Shishido;M. Takeda;Y. Horikawa;H. Matsuoka;A. Yoshida
通讯作者：
A. Yoshida