Microbial communities and symbionts in the hard tick Haemaphysalis longicornis (Acari: Ixodidae) from north China.
Microbial communities and symbionts in the hard tick Haemaphysalis longicornis (Acari: Ixodidae) from north China.
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中国北方硬蜱 Haemaphysalis longicornis（螨虫：蜱科）的微生物群落和共生体
DOI：
10.1186/1756-3305-6-310
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发表时间：
2013-10-28
影响因子：
3.2
通讯作者：
Liu JZ
中科院分区：
文献类型：
--
作者：
Liu LM;Liu JN;Liu Z;Yu ZJ;Xu SQ;Yang XH;Li T;Li SS;Guo LD;Liu JZ
背景：蜱虫与微生物群落之间的密切关系对于蜱虫的适应性以及病原体的定植和传播非常重要。长角血蜱在我国分布广泛，可携带和传播多种病原体，对公众健康和经济造成严重危害。然而，人们对自然条件下长角长角藻中更广泛的微生物群落和共生体知之甚少。在本研究中，我们调查了与长角杆菌相关的细菌群落的组成，并评估了假定的共生体。方法：构建长角杆菌真细菌16S rRNA基因克隆文库，并通过限制性片段长度多态性（RFLP）和DNA测序进行分析。此外，还进行了诊断性PCR，以评估长角长角虫中共生菌的流行情况、垂直传播和感染部位。结果：从现场采集的长角长角角虫中鉴定出垂直传播的共生体、潜在病原体和异源非致病菌。在单一宿主中同时鉴定出三种类型的共生体（类柯克斯体、类砷菌和类立克次体共生体）。一系列分析揭示了这些共生体的垂直传播、流行率和感染部位。然而，只有类柯克斯体细菌在实验室饲养的蜱中稳定传播。此外，我们还发现了一种新型类柯克斯体物质，与之前描述的分类单元具有 95.31% 的序列相似性。结论：本研究表明，天然长角 H. longicornis 拥有多种微生物群落。在单个宿主中同时鉴定出三种类型的共生体。此外，高患病率、垂直传播和感染部位支持柯克斯体共生体与其宿主之间的专性共生关系。柯克斯体共生体在宿主适应性和微生物群落之间相互作用中的作用仍有待阐明。我们对蜱中微生物群落的研究揭示了宿主与微生物之间生态相互作用的复杂性，并为蜱的生物控制提供了见解。
Background:Close relationships between ticks and microbial communities are important for tick fitness and pathogen colonization and transmission. Haemaphysalis longicornis, distributed widely in China, can carry and transmit various pathogens and pose serious damages to public health and economics. However, little is known about the broader array of microbial communities and symbionts in H. longicornis under natural conditions. In the present study, we investigated the composition of bacterial communities associated with H. longicornis and evaluated the putative symbionts.Methods:The eubacterial 16S rRNA gene clone libraries of H. longicornis were constructed and analyzed by restriction fragment length polymorphism (RFLP) and DNA sequencing. In addition, diagnostic PCR was performed to assess the prevalence, vertical transmission and infection sites of the symbionts in H. longicornis.Results:Vertically-transmitted symbionts, potential pathogens and allochthonous nonpathogenic bacteria were identified from the field-collected H. longicornis. Three types of symbionts (Coxiella-like, Arsenophonus-like and Rickettsia-like symbionts) were identified in a single host simultaneously. A series of analyses revealed the vertical transmission, prevalence, and infection sites of these symbionts. However, only Coxiella-like bacteria were transmitted stably in the laboratory-reared ticks. In addition, we identified a novel Coxiella-like agent with 95.31% sequence similarity to the taxon described previously.Conclusions:The present study demonstrated that natural H. longicornis harboured a diverse array of microbial communities. Three types of symbionts were identified in a single host simultaneously. Moreover, high prevalence, vertical transmission and the infection sites supported an obligate symbiotic association between Coxiella symbiont and its host. The role of Coxiella symbiont in the host fitness and the interaction among microbial communities remained to be elucidated. Our investigation of microbial communities in the ticks revealed the complexity of ecological interactions between host and microbe and provided insight for the biological control of ticks.
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影响因子：
1.3
作者：
HOOGSTRAAL, H;ROBERTS, FHS;TIPTON, VJ
通讯作者：
TIPTON, VJ
影响因子：
3.4
作者：
Grindle, N;Tyner, JJ;Fuqua, C
通讯作者：
Fuqua, C
影响因子：
3.2
作者：
Almeida, Aliny P.;Marcili, Arlei;Labruna, Marcelo B.
通讯作者：
Labruna, Marcelo B.
影响因子：
4.4
作者：
Baldridge, GD;Burkhardt, NY;Munderloh, UG
通讯作者：
Munderloh, UG
影响因子：
2.1
作者：
Heise, Stephanie R.;Elshahed, M. S.;Little, S. E.
通讯作者：
Little, S. E.