Characterization and bioactivity of polysaccharides obtained from pine cones of Pinus koraiensis by graded ethanol precipitation.
Characterization and bioactivity of polysaccharides obtained from pine cones of Pinus koraiensis by graded ethanol precipitation.
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分级乙醇沉淀红松球果多糖的表征及生物活性
DOI：
10.3390/molecules18089933
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发表时间：
2013-08-19
期刊：
影响因子：
--
通讯作者：
Qin D
中科院分区：
文献类型：
--
作者：
Zou P;Yang X;Huang WW;Zhao HT;Wang J;Xu RB;Hu XL;Shen SY;Qin D
采用热水浸提法从红松球果中提取红松多糖。在乙醇浓度分别为30%、50%、60%、70%和80%的条件下，成功分离了5个多糖组分，分别命名为PKP-A、PKP-B、PKP-C、PKP-D和PKP-E。采用高效液相色谱法、红外光谱法、气相色谱-质谱联用仪和氨基酸自动分析仪对其化学性质进行了研究。单糖组成分析表明，5个组分均由d-核糖、l-鼠李糖、l-阿拉伯糖、d-木糖、d-甘露糖、d-葡萄糖和d-半乳糖组成，但它们的摩尔比有较大差异。HPLC结果表明，较高浓度乙醇沉淀的多糖分子量较低。采用羟自由基和ABTS自由基清除试验研究了五个组分的抗氧化活性。5个分级多糖组分均表现出较好的抑制作用，体外MTT法测定PKP-A和PKP-E的IC 50分别为1，072.5和2，070.0 μg·mL-1。这些结果表明，PKP可能是天然抗氧化剂或膳食补充剂的潜在来源。
Pinus koraiensis polysaccharides (PKP) were extracted by hot water from P. koraiensis pine cones. Five polysaccharide fractions named PKP-A, PKP-B, PKP-C, PKP-D and PKP-E were successfully separated at final ethanol concentrations of 30%, 50%, 60%, 70% and 80%, respectively. HPLC, FT-IR, GC-MS and automatic amino-acid analysis were applied to investigate their chemical characteristics. Monosaccharide component analysis indicated that the five fractions were all composed of d-ribose, l-rhamnose, l-arabinose, d-xylose, d-mannose, d-glucose and d-galactose, but their molar ratios were quite different. HPLC results revealed that the polysaccharides precipitated by higher concentrations of ethanol solution had lower molecular masses. Moreover, the antioxidant activities of the five fractions were studied on the basis of hydroxyl radical and ABTS radical scavenging tests. The five graded polysaccharide fractions exhibited good inhibitory power, and MTT tests in vitro showed the IC50 of PKP-A and PKP-E were 1,072.5 and 2,070.0 μg·mL−1, respectively. These results demonstrated that the PKP could be a potential source of natural antioxidants or dietary supplements.
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