Isolation and expression analysis of three EIN3-like genes in tree peony (Paeonia suffruticosa)-猫眼课题宝

牡丹中3个EIN3样基因的分离及表达分析

DOI：

10.1007/s11240-012-0220-z

发表时间：

2012-08

期刊：

Plant Cell, Tissue and Organ Culture

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

Wang, Yanjie;Zhang, Chao;Jia, Peiyi;Wang, Xiaoqing;Wang, Weiran;Dong, Li

Ethylene is a critical signal that influences cut flower opening and senescence in tree peony (Paeonia suffruticosa), which is a traditional ornamental plant in China.ArabidopsisETHYLENE-INSENSITIVE3 (EIN3) acts as a key transcription factor of the ethylene signaling pathway, suggesting a possible role for its homologues in regulation of cut flower postharvest development. In this study, three EIN3 homologous genes includingPsEIL1,PsEIL2, andPsEIL3have been isolated from petals of tree peony. Deduced amino acid sequences of conserved domains ofPsEILsshare high similarities with theArabidopsisEIN3 protein. Real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that threePsEILswere differentially expressed in various tissues of tree peony, and none was flower specific. During cut flower postharvest development,PsEIL1transcript in petals accumulated at a relatively low level at the opening stage and reached the highest level of mRNA accumulation at senescence;PsEIL2andPsEIL3transcripts were gradually increased and peaked at full opening stage followed by a decline when petals wilted. However, thesePsEILgenes exhibited differential responses to ethylene and 1-methycyclopropene (1-MCP) treatments. Compared with the control, the mRNA level ofPsEIL1was not influenced by either ethylene or 1-MCP treatment, whereas bothPsEIL2andPsEIL3transcripts were significantly increased by exogenous ethylene, and onlyPsEIL3was strongly inhibited by 1-MCP. These results suggest thatPsEILtranscripts are spatiotemporally regulated, and the transcriptional regulation ofPsEIL3may play an important role in ethylene-mediated cut flower opening and senescence in tree peony.