Experimental validation and pan-cancer analysis identified COL10A1 as a novel oncogene and potential therapeutic target in prostate cancer.
Experimental validation and pan-cancer analysis identified COL10A1 as a novel oncogene and potential therapeutic target in prostate cancer.
复制标题
DOI：
10.18632/aging.205337
复制
发表时间：
2023-12-21
期刊：
影响因子：
5.2
通讯作者：
Zhang, Hongtuan
中科院分区：
文献类型：
--
作者：
Xu, Shengxian;Liu, Dongze;Qin, Zheng;Liang, Zhengxin;Xie, Hongbo;Yi, Bocun;Wang, Kaibin;Lin, Gaoteng;Liu, Ranlu;Yang, Kuo;Xu, Yong;Zhang, Hongtuan
背景资料：X型胶原（COL10）是在人体组织的细胞外基质中发现的同源三聚体非纤维状胶原，并且其表现出独特的白色外观。X型胶原α1链（COL10A1）是X型胶原的特异性裂解片段。然而，COL10A1在前列腺癌和泛癌背景中的表达、预后意义、临床病理学属性和免疫相关性仍然知之甚少。研究方法：使用来自最新数据库（TCGA，GTEx和GEO数据库）的数据的生物信息学分析，我们已经广泛阐明了COL10A1在其表达模式，预后意义和免疫功效方面的作用。随后，通过实验验证阐明了COL10A1在前列腺癌中的生物学功能。结果：我们的研究结果证实，COL10A1在大多数癌症中高表达，并与癌症患者的预后不良相关。免疫相关性分析显示，COL10A1与肿瘤突变负荷（TMB）、微卫星不稳定性（MSI）和免疫细胞浸润显著相关。此外，前列腺癌中COL10A1的敲低导致前列腺癌细胞的增殖、迁移和侵袭潜力显著降低。结论：我们对COL10A1基因的泛癌分析为其在癌症发生，进展和治疗意义中的关键作用提供了新的见解，强调了其在癌症，特别是前列腺癌的预后和免疫干预中的潜在意义。
Background: Type X collagen (COL10) is a homologous trimeric non-fibrillar collagen found in the extracellular matrix of human tissues, and it exhibits a distinctive white appearance. Type X collagen α1 chain (COL10A1) is a specific cleaved fragment of type X collagen. However, the expression, prognostic significance, clinicopathological attributes and immune-related associations of COL10A1 in prostate cancer as well as in pan-cancer contexts remain poorly understood. Methods: Using bioinformatic analysis of data from the most recent databases (TCGA, GTEx and GEO databases), we have extensively elucidated the role played by COL10A1 in terms of its expression patterns, prognostic implications, and immune efficacy across a pan-cancer spectrum. Subsequently, the biological functions of COL10A1 in prostate cancer were elucidated by experimental validation. Results: Our findings have confirmed that COL10A1 was highly expressed in most cancers and was associated with poorer prognosis in cancer patients. Immune correlation analysis of COL10A1 in various cancers showed its significant correlation with Tumor mutational burden (TMB), microsatellite instability (MSI) and immune cell infiltration. In addition, knockdown of COL10A1 in prostate cancer resulted in a substantial reduction in the proliferation, migration, and invasive potential of prostate cancer cells. Conclusion: Our pan-cancer analysis of COL10A1 gene provided novel insights into its pivotal role in cancer initiation, progression, and therapeutic implications, underscoring its potential significance in prognosis and immunotherapeutic interventions for cancer, particularly prostate cancer.
登录
查看更多内容
影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
COL10A1高表达与结直肠癌不良预后相关
影响因子：
4
作者：
Huang H;Li T;Ye G;Zhao L;Zhang Z;Mo D;Wang Y;Zhang C;Deng H;Li G;Liu H
通讯作者：
Liu H
影响因子：
8.8
作者：
Ino Y;Yamazaki-Itoh R;Shimada K;Iwasaki M;Kosuge T;Kanai Y;Hiraoka N
通讯作者：
Hiraoka N
影响因子：
7.3
作者：
Liu Z;Lu T;Li J;Wang L;Xu K;Dang Q;Liu L;Guo C;Jiao D;Sun Z;Han X
通讯作者：
Han X
TGF-β1-SOX9轴诱导的COL10A1通过上皮间质转化促进胃癌侵袭和转移
影响因子：
9
作者：
Li T;Huang H;Shi G;Zhao L;Li T;Zhang Z;Liu R;Hu Y;Liu H;Yu J;Li G
通讯作者：
Li G