Surface chemistry but not aspect ratio mediates the biological toxicity of gold nanorods in vitro and in vivo.
Surface chemistry but not aspect ratio mediates the biological toxicity of gold nanorods in vitro and in vivo.
复制标题
表面化学而非纵横比介导金纳米棒的体外和体内生物毒性。
DOI：
10.1038/srep11398
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发表时间：
2015-06-22
影响因子：
4.6
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Wan J;Wang JH;Liu T;Xie Z;Yu XF;Li W
金纳米棒是一种很有前途的纳米材料，在临床诊断和治疗中具有广阔的应用前景。GNRs的大小、形状和表面特征等物理化学性质是影响其细胞毒性的关键因素。在这项研究中，我们研究了不同的长宽比和表面修饰对培养细胞和小鼠体内GNRs的细胞毒性和细胞摄取的影响。结果表明，GNRs的生物毒性是由其表面化学作用而不是长径比决定的。不同长径比的CTAB-GNRs通过损伤线粒体和激活细胞内活性氧(ROS)，诱导细胞凋亡和自噬的能力相似。而CTAB/PSS、CTAB/PAH、CTAB/PSS/PAH或CTAB/PAH/PSS包衣GNRs的毒性较低，且不诱导细胞死亡。与CTAB/PSS和CTAB/PSS/PAH包衣GNRs相比，CTAB/PAH包衣GNRs对细胞形态的影响最小。此外，静脉注射CTAB/PAH GNRs使GNRs能够通过动物的血液循环到达肿瘤组织，并保持稳定，与其他GNRs相比，半衰期更长。因此，我们的结果表明，与改变GNR的长径比类似，CTAB/PAH包衣GNR的进一步包膜可以防止细胞毒性和细胞死亡，并且CTAB/PAH包衣GNR具有更好的生物相容性和最低的细胞毒性。
Gold nanorods are a promising nanoscale material in clinical diagnosis and treatment. The physicochemical properties of GNRs, including size, shape and surface features, are crucial factors affecting their cytotoxicity. In this study, we investigated the effects of different aspect ratios and surface modifications on the cytotoxicity and cellular uptake of GNRs in cultured cells and in mice. The results indicated that the surface chemistry but not the aspect ratio of GNRs mediates their biological toxicity. CTAB-GNRs with various aspect ratios had similar abilities to induce cell apoptosis and autophagy by damaging mitochondria and activating intracellular reactive oxygen species (ROS). However, GNRs coated with CTAB/PSS, CTAB/PAH, CTAB/PSS/PAH or CTAB/PAH/PSS displayed low toxicity and did not induce cell death. CTAB/PAH-coated GNRs caused minimally abnormal cell morphology compared with CTAB/PSS and CTAB/PSS/PAH coated GNRs. Moreover, the intravenous injection of CTAB/PAH GNRs enabled the GNRs to reach tumor tissues through blood circulation in animals and remained stable, with a longer half-life compared to the other GNRs. Therefore, our results demonstrated that further coating can prevent cytotoxicity and cell death upon CTAB-coated GNR administration, similar to changing the GNR aspect ratio and CTAB/PAH coated GNRs show superior biological properties with better biocompatibility and minimal cytotoxicity.
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影响因子：
3.3
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通讯作者：
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