Molecular cloning and expression of a new gene, GON-SJTU1 in the rat testis.
Molecular cloning and expression of a new gene, GON-SJTU1 in the rat testis.
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新基因GON-SJTU1的分子克隆及在大鼠睾丸中的表达
DOI：
10.1186/1477-7827-8-43
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发表时间：
2010-05-12
期刊：
影响因子：
--
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Yang ZJ;Sun N;Wang SQ;Tian GG;Wu J
精子发生是一个涉及细胞发育、分化和凋亡的复杂过程。这个过程是由一系列表达受到高度调控的基因控制的。男性不育可归因于与精子发生有关的多种遗传缺陷或改变。精子发生相关基因的发现、克隆及其功能的进一步研究，对阐明精子发生的分子机制具有重要意义。它对男性不育的治疗也具有生理和病理意义。通过cDNA文库筛选和3′和5′-RACE技术，从大鼠睾丸中鉴定并克隆了GON-SJTU1。采用Northern blotting和Western blotting对GON-SJTU1的产物进行评价。采用原位杂交和免疫组织化学方法检测GON-SJTU1的表达。本研究鉴定并克隆了一个具有精子发生生物学过程的新基因GON-SJTU1。从第1天到第15天，GON-SJTU1在睾丸中高表达，然后下降，提示GON-SJTU1可能是一个与时间相关的基因，参与了精子发生的早期阶段。GON-SJTU1在睾丸中的表达出现在一些男性生殖细胞中，特别是在性腺细胞和精原干细胞中。GON-SJTU1可能在精子发生的生物学过程中发挥作用。
Spermatogenesis is a complex process involving cell development, differentiation and apoptosis. This process is governed by a series of genes whose expressions are highly regulated. Male infertility can be attributed to multiple genetic defects or alterations that are related to spermatogenesis. The discovery, cloning and further functional study of genes related to spermatogenesis is of great importance to the elucidation of the molecular mechanism of spermatogenesis. It is also physiologically and pathologically significant to the therapy of male infertility. GON-SJTU1 was identified and cloned from rat testis by cDNA library screening and 3'-and 5'-RACE. The products of GON-SJTU1 were assessed by Northern and Western blotting. The expression of GON-SJTU1 was also examined by In situ hybridization and immunohistochemistry. Here we identified and cloned a new gene, GON-SJTU1, with the biological process of spermatogenesis. GON-SJTU1 is highly expressed in the testis from day 1 to 15 and then decreased, suggesting that GON-SJTU1 might be a time-related gene and involved in the early stage of spermatogenesis. And the expression of GON-SJTU1 in the testis occurred in some male germ cells, particularly in gonocytes and spermatogonial stem cells. GON-SJTU1 may play a role in the biological process of spermatogenesis.
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影响因子：
10.5
作者：
Harrison, DA;McCoon, PE;Perrimon, N
通讯作者：
Perrimon, N
影响因子：
3.6
作者：
DYM, M;JIA, MC;RAVINDRANATH, N
通讯作者：
RAVINDRANATH, N
影响因子：
13.3
作者：
Mauduit, C;Hamamah, S;Benahmed, M
通讯作者：
Benahmed, M
DOI：
10.1073/pnas.0709854105
发表时间：
2008-06-17
影响因子：
11.1
作者：
Johnston, Daniel S.;Wright, William W.;Jelinsky, Scott A.
通讯作者：
Jelinsky, Scott A.
影响因子：
2.7
作者：
Hombría, JCG;Brown, S;Zeidler, MP
通讯作者：
Zeidler, MP