Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator.
Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator.
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DOI：
10.1371/journal.pone.0040003
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Xi P
中科院分区：
文献类型：
--
作者：
Liu Y;Ding Y;Alonas E;Zhao W;Santangelo PJ;Jin D;Piper JA;Teng J;Ren Q;Xi P
In this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. With synchronization-free STED, we imaged 200 nm nanospheres as well as all three cytoskeletal elements (microtubules, intermediate filaments, and actin filaments), clearly demonstrating the resolving power of synchronization-free STED over conventional diffraction limited imaging. It also allowed us to discover that, Dylight 650, exhibits improved performance over ATTO647N, a fluorophore frequently used in STED. Furthermore, we applied synchronization-free STED to image fluorescently-labeled intracellular viral RNA granules, which otherwise cannot be differentiated by confocal microscopy. Thanks to the widely available Ti:Sapphire oscillators in multiphoton imaging system, this work suggests easier access to setup super-resolution microscope via the synchronization-free STED.
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