The challenge of determining handedness in electron tomography and the use of DNA origami gold nanoparticle helices as molecular standards.

The challenge of determining handedness in electron tomography and the use of DNA origami gold nanoparticle helices as molecular standards.
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DOI:
10.1016/j.jsb.2013.04.008
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发表时间:
2013-07
影响因子:
3
通讯作者:
Jensen, Grant J.
Jensen, Grant J.
中科院分区:
生物学3区
文献类型:
--
作者:
Briegel, Ariane;Pilhofer, Martin;Mastronarde, David N.;Jensen, Grant J.

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电磁层析成像重建的明显旋向性取决于许多惯例,并且可以在许多方面混淆。随着用于电子断层扫描的不同硬件和软件组合的数量不断攀升,并且所产生的重建达到越来越高的分辨率,验证结果的手的需求已经增加。在这里,我们列举了一个典型的断层扫描实验中的各种步骤,影响偏手性和DNA折纸金纳米粒子螺旋可以用作方便和故障安全的偏手性标准。
The apparent handedness of an EM-tomography reconstruction depends on a number of conventions and can be confused in many ways. As the number of different hardware and software combinations being used for electron tomography continue to climb, and the reconstructions being produced reach higher and higher resolutions, the need to verify the hand of the results has increased. Here we enumerate various steps in a typical tomography experiment that affect handedness and show that DNA origami gold nanoparticle helices can be used as convenient and fail-safe handedness standards.
DOI: 10.1006/jsbi.1997.3896
发表时间: 1997-01-01
影响因子: 3
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