The challenge of determining handedness in electron tomography and the use of DNA origami gold nanoparticle helices as molecular standards.
The challenge of determining handedness in electron tomography and the use of DNA origami gold nanoparticle helices as molecular standards.
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DOI:
10.1016/j.jsb.2013.04.008
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发表时间:
2013-07
影响因子:
3
通讯作者:
Jensen, Grant J.
中科院分区:
文献类型:
--
作者:
Briegel, Ariane;Pilhofer, Martin;Mastronarde, David N.;Jensen, Grant J.
The apparent handedness of an EM-tomography reconstruction depends on a number of conventions and can be confused in many ways. As the number of different hardware and software combinations being used for electron tomography continue to climb, and the reconstructions being produced reach higher and higher resolutions, the need to verify the hand of the results has increased. Here we enumerate various steps in a typical tomography experiment that affect handedness and show that DNA origami gold nanoparticle helices can be used as convenient and fail-safe handedness standards.
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影响因子:
3
作者:
Belnap, DM;Olson, NH;Baker, TS
通讯作者:
Baker, TS
影响因子:
19
作者:
McIntosh, R;Nicastro, D;Mastronarde, D
通讯作者:
Mastronarde, D
DOI:
10.1002/jemt.1060090405
发表时间:
1988-08-01
期刊:
JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE
影响因子:
--
作者:
RADERMACHER, M
通讯作者:
RADERMACHER, M
DOI:
10.1073/pnas.1115719109
发表时间:
2012-03-06
影响因子:
11.1
作者:
Briegel, Ariane;Li, Xiaoxiao;Crane, Brian R.
通讯作者:
Crane, Brian R.
影响因子:
64.8
作者:
Kuzyk, Anton;Schreiber, Robert;Liedl, Tim
通讯作者:
Liedl, Tim