Non-invasive assessment of elastic modulus of arterial constructs during cell culture using ultrasound elasticity imaging.
Non-invasive assessment of elastic modulus of arterial constructs during cell culture using ultrasound elasticity imaging.
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DOI：
10.1016/j.ultrasmedbio.2013.04.023
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发表时间：
2013-11
影响因子：
2.9
通讯作者：
Kim, Kang
中科院分区：
文献类型：
--
作者：
Dutta, Debaditya;Lee, Kee-Won;Allen, Robert A.;Wang, Yadong;Brigham, John C.;Kim, Kang
关键词：
机械强度是工程动脉的关键设计因素。大多数现有的技术评估动脉结构的机械性能破坏性，导致大量的动物牺牲。我们提出了一种基于超声的无创机械强度评估技术的工程动脉结构。在脉动流生物反应器中培养由生物可降解弹性体制成并接种有血管成纤维细胞和平滑肌细胞的管状支架。通过二维相敏散斑跟踪从脉动支架的超声射频信号计算支架扩张。然后通过从扩张和记录的脉压求解逆问题来计算杨氏模量。因此，从超声波计算的刚度与直接的机械测试结果相关。随着支架在培养中成熟，超声测量显示杨氏模量增加，组织学证实了结构中细胞和胶原纤维的生长。结果表明，超声弹性成像非侵入性评估和监测动脉结构的机械性能。
Mechanical strength is a key design factor for engineered arteries. Most existing techniques assess the mechanical property of arterial constructs destructively, leading to a large number of animal sacrifices. We propose an ultrasound-based non-invasive mechanical strength assessment technique for engineered arterial constructs. Tubular scaffolds made from a biodegradable elastomer and seeded with vascular fibroblasts and smooth muscle cells were cultured in a pulsatile-flow bioreactor. Scaffold distension was computed from ultrasound radiofrequency signals of the pulsating scaffold via two-dimensional phase-sensitive speckle tracking. The Young's modulus was then calculated by solving inverse problem from the distension and the recorded pulse pressure. Stiffness thus computed from ultrasound correlated well with direct mechanical testing results. As the scaffolds matured in culture, ultrasound measurements showed increased Young's modulus and histology confirmed the growth of cells and collagen fibrils in the constructs. The results show that ultrasound elastography non-invasively assesses and monitors the mechanical properties of arterial constructs.
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影响因子：
4.9
作者：
Gao, Jin;Ensley, Ann E.;Wang, Yadong
通讯作者：
Wang, Yadong
影响因子：
4.9
作者：
Gao, Jin;Crapo, Peter;Wang, Yadong
通讯作者：
Wang, Yadong
影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
DOI：
10.1073/pnas.0907813106
发表时间：
2010-02-23
影响因子：
11.1
作者：
Niklason, Laura E.;Yeh, Alvin T.;Humphrey, Jay D.
通讯作者：
Humphrey, Jay D.
影响因子：
14
作者：
Levitz D;Hinds MT;Ardeshiri A;Hanson SR;Jacques SL
通讯作者：
Jacques SL