Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential.
Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential.
复制标题
胫骨前肌和提腭肌的形态和分子比较：增强潜力的初步研究
DOI：
10.3892/etm.2017.5391
复制
发表时间：
2018-01
影响因子：
2.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Cheng X;Song L;Lan M;Shi B;Li J
胫骨前肌（TA）和其他somite衍生的肢体肌肉仍然是骨骼肌研究的原型。然而，大多数头部肌肉是从鳃弓发育而来的，与四肢肌肉相比，头部肌肉具有一定的异质性。提腭veli palatini （LVP）肌是位于头部深处的肌肉，负责呼吸、吞咽和语言，是腭裂手术的核心，但缺乏形态学和分子研究。在本研究中，进行了多尺度体内分析，比较TA和LVP肌肉的肌纤维组成、原位干细胞群和增强潜力。TA肌主要由2B型肌纤维组成，而LVP肌主要由2A型和2X型肌纤维组成。此外，LVP肌保持了较高比例的中心有核肌纤维和更多的卫星细胞。值得注意的是，TA和LVP肌肉对外源性Wnt7a刺激的反应方式不同。Wnt7a给药3周后，TA肌肌纤维数量增加，肌纤维大小减小，而LVP肌肌纤维数量和肌纤维大小无明显变化。这些结果表明，与TA肌相比，LVP肌表现出明显的差异。因此，从TA肌肉研究中获得的知识在应用于LVP肌肉之前需要进一步的测试。
Tibialis anterior (TA) muscle and other somite-derived limb muscles remain the prototype in skeletal muscle study. The majority of head muscles, however, develop from branchial arches and maintain a number of heterogeneities in comparison with their limb counterparts. Levator veli palatini (LVP) muscle is a deep-located head muscle responsible for breathing, swallowing and speech, and is central to cleft palate surgery, yet lacks morphological and molecular investigation. In the present study, multiscale in vivo analyses were performed to compare TA and LVP muscle in terms of their myofiber composition, in-situ stem cell population and augmentation potential. TA muscle was identified to be primarily composed of type 2B myofibers while LVP muscle primarily consisted of type 2A and 2X myofibers. In addition, LVP muscle maintained a higher percentage of centrally-nucleated myofibers and a greater population of satellite cells. Notably, TA and LVP muscle responded to exogenous Wnt7a stimulus in different ways. Three weeks after Wnt7a administration, TA muscle exhibited an increase in myofiber number and a decrease in myofiber size, while LVP muscle demonstrated no significant changes in myofiber number or myofiber size. These results suggested that LVP muscle exhibits obvious differences in comparison with TA muscle. Therefore, knowledge acquired from TA muscle studies requires further testing before being applied to LVP muscle.
登录
查看更多内容
软pa的大鼠肌肉再生模型。
影响因子：
3.7
作者：
Carvajal Monroy PL;Grefte S;Kuijpers-Jagtman AM;Helmich MP;Ulrich DJ;Von den Hoff JW;Wagener FA
通讯作者：
Wagener FA
影响因子：
2.7
作者：
Stuelsatz, Pascal;Shearer, Andrew;Li, Yunfei;Muir, Lindsey A.;Ieronimakis, Nicholas;Shen, Qingwu W.;Kirillova, Irina;Yablonka-Reuveni, Zipora
通讯作者：
Yablonka-Reuveni, Zipora
影响因子：
5.8
作者：
Dumont, Nicolas A.;Bentzinger, C. Florian;Rudnicki, Michael A.
通讯作者：
Rudnicki, Michael A.
影响因子：
6.4
作者：
Monroy, Paola L. Carvajal;Grefte, Sander;Von den Hoff, Johannes W.
通讯作者：
Von den Hoff, Johannes W.
DOI：
10.1073/pnas.0701122104
发表时间：
2007-04-03
影响因子：
11.1
作者：
Shih, Hung Ping;Gross, Michael K.;Kioussi, Chrissa
通讯作者：
Kioussi, Chrissa