Increasing the strength and bioactivity of collagen scaffolds using customizable arrays of 3D-printed polymer fibers.
Increasing the strength and bioactivity of collagen scaffolds using customizable arrays of 3D-printed polymer fibers.
复制标题
DOI：
10.1016/j.actbio.2016.02.004
复制
发表时间：
2016-03
影响因子：
9.7
通讯作者：
Harley, Brendan A. C.
中科院分区：
文献类型：
--
作者：
Mozdzen, Laura C.;Rodgers, Ryan;Banks, Jessica M.;Bailey, Ryan C.;Harley, Brendan A. C.
肌腱是一种高度定向的结缔组织，它将力从肌肉传递到骨骼。每年，美国有超过3200万人遭受肌腱损伤。为了减轻因瘢痕形成导致的不良功能结果，目前的手术技术在很大程度上依赖于自体移植。生物材料平台和组织工程方法为解决这些损伤提供了一种替代方法。包含定向结构特征的支架可以促进能够向肌腱细胞分化的成年肌腱细胞和间充质干细胞的扩增。然而，在这些构建体的支架生物活性和机械强度之间取得适当平衡仍然具有挑战性。三维构建体中为促进细胞浸润、营养物质和氧气的生物转运所需的高孔隙率通常导致生物力学强度不足。在此，我们描述了使用三维打印技术来创建丙烯腈 - 丁二烯 - 苯乙烯（ABS）纤维的可定制阵列，这些纤维可被纳入正在开发的用于肌腱修复的胶原蛋白支架中。值得注意的是，通过改变纤维增强的几何形状，可以有选择地控制支架 - 纤维复合材料的力学性能（弹性模量、峰值应力、峰值应力下的应变和韧性），而不影响胶原蛋白支架的天然生物活性。此外，我们报告了一种通过顺序的氧等离子体和碳二亚胺交联处理用诱导活性的生长因子使ABS纤维功能化的方法。总之，我们报告了一种适应性方法，以一种与胶原蛋白支架本身的结构正交的方式来控制机械强度和生物分子信号的存在。
Tendon is a highly aligned connective tissue which transmits force from muscle to bone. Each year, people in the US sustain more than 32 million tendon injuries. To mitigate poor functional outcomes due to scar formation, current surgical techniques rely heavily on autografts. Biomaterial platforms and tissue engineering methods offer an alternative approach to address these injuries. Scaffolds incorporating aligned structural features can promote expansion of adult tenocytes and mesenchymal stem cells capable of tenogenic differentiation. However, appropriate balance between scaffold bioactivity and mechanical strength of these constructs remains challenging. The high porosity required to facilitate cell infiltration, nutrient and oxygen biotransport within three-dimensional constructs typically results in insufficient biomechanical strength. Here we describe the use of three-dimensional printing techniques to create customizable arrays of acrylonitrile butadiene styrene (ABS) fibers that can be incorporated into a collagen scaffold under development for tendon repair. Notably, mechanical performance of scaffold-fiber composites (elastic modulus, peak stress, strain at peak stress, and toughness) can be selectively manipulated by varying fiber-reinforcement geometry without affecting the native bioactivity of the collagen scaffold. Further, we report an approach to functionalize ABS fibers with activity-inducing growth factors via sequential oxygen plasma and carbodiimide crosslinking treatments. Together, we report an adaptable approach to control both mechanical strength and presence of biomolecular cues in a manner orthogonal to the architecture of the collagen scaffold itself.
登录
查看更多内容
影响因子：
14
作者：
Banks, Jessica M.;Mozdzen, Laura C.;Harley, Brendan A. C.;Bailey, Ryan C.
通讯作者：
Bailey, Ryan C.
影响因子：
10
作者：
Caliari, Steven R.;Weisgerber, Daniel W.;Grier, William K.;Mahmassani, Ziad;Boppart, Marni D.;Harley, Brendan A. C.
通讯作者：
Harley, Brendan A. C.
用于肌腱组织工程的胶原蛋白 - 胶原型支架膜复合材料的发展。
影响因子：
14
作者：
Caliari SR;Ramirez MA;Harley BA
通讯作者：
Harley BA
影响因子：
2.8
作者：
Galatz, LM;Sandell, LJ;Thomopoulos, S
通讯作者：
Thomopoulos, S
影响因子：
5.3
作者：
Chamberlain, LJ;Yannas, IV;Spector, M
通讯作者：
Spector, M