QTL mapping under salt stress in rice using a Kalarata-Azucena population.
QTL mapping under salt stress in rice using a Kalarata-Azucena population.
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DOI：
10.1007/s10681-022-03026-8
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发表时间：
2022
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--
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--
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由于现代水稻品种的高度敏感性，盐胁迫是亚洲大型水稻产区的主要限制因素。为了鉴定与水稻耐盐性相关的数量性状基因座（QTL），我们从耐盐的地方品种Kalarata和盐敏感的亲本Azucena之间的杂交F2群体开发。从这个群体的F3家系筛选和耐盐性评分使用IRRI的标准评价系统（SES）。生长，生物量，Na+和K+浓度在叶组织中，叶绿素浓度进行了测定。利用151个SSR标记和InDel标记构建了一张遗传连锁图谱，图谱覆盖范围为1463 cM，位点间平均距离为9.69cM。利用复合区间作图法对16个性状共定位到13个QTL。在本研究中发现了几个新的QTL，其中最大的是位于第3染色体上的根钠浓度QTL（LOD = 11.0，R2 = 25.0），它也与SES QTL共定位。第1染色体短臂上的多个QTL与之前鉴定的Saltol位点一致。本研究中发现的新QTL构成了未来分子育种的目标，将其与之前发现的其他QTL联合收割机结合，以提高水稻品种在盐害土壤中的耐受性和稳定表现。在线版本包含补充材料，可通过10.1007/s10681-022-03026-8获得。
Salt stress is a major constraint across large rice production areas in Asia, because of the high sensitivity of modern rice varieties. To identify quantitative trait loci (QTL) associated with salt tolerance in rice, we developed an F2 population from a cross between the salt-tolerant landrace, Kalarata, and the salt-sensitive parent, Azucena. F3 families from this population were screened and scored for salt tolerance using IRRI’s Standard evaluation system (SES). Growth, biomass, Na+ and K+ concentrations in leaf tissues, and chlorophyll concentration were determined. A genetic linkage map was constructed with 151 SSRs and InDel markers, which cover 1463 cM with an average distance of 9.69 cM between loci. A total of 13 QTL were identified using Composite Interval Mapping for 16 traits. Several novel QTL were identified in this study, the largest is for root sodium concentration (LOD = 11.0, R2 = 25.0) on chromosome 3, which also co-localize with a QTL for SES. Several QTL on the short arm of chromosome 1 coincide with the Saltol locus identified before. The novel QTL identified in this study constitute future targets for molecular breeding, to combine them with other QTL identified before, for higher tolerance and stable performance of rice varieties in salt affected soils. The online version contains supplementary material available at 10.1007/s10681-022-03026-8.
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