Fine mapping of qSKC-1, a major quantitative trait locus for shoot K(+) concentration, in rice seedlings grown under salt stress.
Fine mapping of qSKC-1, a major quantitative trait locus for shoot K(+) concentration, in rice seedlings grown under salt stress.
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DOI：
10.1270/jsbbs.16190
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发表时间：
2017-06
期刊：
影响因子：
2.4
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Jing W;Deng P;Cao C;Zhang W
地上部K+浓度（SKC）是评价水稻苗期耐盐性的重要生理指标。以水稻品种“日本晴”及其盐敏感突变体（rss 2和rss 4）与籼稻品种“窄叶青8号”（ZYQ 8）杂交构建的3个F2群体为材料，在第1染色体上检测到盐胁迫下水稻SKC的主要数量性状位点qSKC-1。在这项研究中，qSKC-1的染色体定位精确地确定了精细定位。首先，通过对构建的'日本晴' × 'ZYQ 8' F2群体进行QTL分析，证实了qSKC-1的存在。然后，从“日本晴”דZYQ 8”和rss 4 דZYQ 8”F2群体中鉴定qSKC-1附近发生重组事件的F2植株，并通过后代测定确认其表型值。最后，通过分析这些重组体中的重组事件，qSKC-1基因座被精确定位到标记RM 578和IM 8854之间的445 kb处。这些结果将有助于qSKC-1基因座的图位克隆。
Shoot K+ concentration (SKC) is an important physiological parameter used to evaluate salt tolerance at the seedling stage in rice (Oryza sativa L.). qSKC-1, a major quantitative trait locus for SKC in rice under salt stress, was detected on chromosome 1 using three F2 populations constructed by crossing ‘Nipponbare’ and its two salt-sensitive mutants (rss2 and rss4) with an indica cultivar ‘Zhaiyeqing8’ (‘ZYQ8’). In this study, the chromosomal location of qSKC-1 was determined precisely by fine mapping. First, the presence of qSKC-1 was confirmed by QTL analysis of a re-constructed ‘Nipponbare’ × ‘ZYQ8’ F2 population. Then, F2 plants in which recombination events had occurred near qSKC-1 were identified from the ‘Nipponbare’ × ‘ZYQ8’ and rss4 × ‘ZYQ8’ F2 populations, and their phenotypic values were confirmed by progeny tests. Eventually, by analyzing recombination events in these recombinants, the qSKC-1 locus was mapped precisely to 445 kb between markers RM578 and IM8854. These results will facilitate map-based cloning of the qSKC-1 locus.
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