SOLD1 is expressed in bovine trophoblast cell lines and regulates cell invasiveness.
SOLD1 is expressed in bovine trophoblast cell lines and regulates cell invasiveness.
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DOI：
10.1186/1477-7827-12-55
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发表时间：
2014-06-21
期刊：
影响因子：
--
通讯作者：
Hashizume K
中科院分区：
文献类型：
--
作者：
Awad M;Koshi K;Kizaki K;Takahashi T;Hashizume K
Ly-6结构域1分泌蛋白（SOLD 1）是Ly-6超家族的一个分泌型成员，在整个妊娠期间在胎儿和母体组织中表达。SOLD 1 mRNA在子宫内膜和滋养层单核细胞和双核细胞中表达，表明其不仅在妊娠早期的胎盘结构中起重要作用，而且在妊娠晚期的子宫内膜重塑中也起重要作用。在这里，我们调查的表达SOLD 1 mRNA和蛋白质在滋养层细胞系。此外，我们还研究了SOLD 1对滋养层细胞侵袭能力的影响。我们用定量逆转录PCR（qRT-PCR）检测了13个牛滋养层细胞系中SOLD 1基因的表达。用Western blotting和免疫细胞化学方法检测两种细胞系BT-C和BT-K中SOLD 1蛋白的表达。此外，我们测量了在存在或不存在抗牛SOLD 1抗体的情况下BT细胞的侵袭活性。在可变的水平，SOLD 1在所有13个细胞系中表达，但是，表达仍然低于近端胎膜组织。SOLD 1蛋白，其大小约为28 kDa，在BT细胞的细胞质的核周区域中检测到。用抗牛SOLD 1抗体处理BT-K细胞系的侵袭性具有剂量依赖性抑制作用。本研究是第一个在体外研究SOLD 1在滋养层细胞系中的表达。我们的数据表明，SOLD 1参与调节滋养层细胞的侵袭性。因此，SOLD 1可能在调解母胎界面的沟通中发挥积极和关键的作用。
Secreted protein of Ly-6 domain 1 (SOLD1), a secretory-type member of the Ly-6 superfamily, is expressed in both fetal and maternal tissues throughout gestation. SOLD1 mRNA is expressed in the endometrium and in trophoblast mononucleate and binucleate cells, suggesting it plays an important role not only in placental architecture at early gestation, but also in remodeling the endometrium at late gestation. Here, we investigate the expression of SOLD1 mRNA and protein in trophoblast cell lines. In addition, we examine the effect of SOLD1 on the invasive ability of trophoblast cells. We measured SOLD1 gene expression in thirteen bovine trophoblast (BT) cell lines by using quantitative reverse transcription PCR (qRT-PCR). SOLD1 protein levels were examined in two cell lines, BT-C and BT-K, by using Western blotting and immunocytochemistry. In addition, we measured the invasive activity of BT cells in the presence or absence of anti-bovine SOLD1 antibodies. At variable levels, SOLD1 was expressed in all thirteen cell lines; however, expression remained below that of proximal fetal membrane tissue. SOLD1 protein, which was approximately 28 kDa in size, was detected in perinuclear area of the cytoplasm in BT cells. Treatment with anti-bovine SOLD1 antibody had a dose-dependent suppressive effect on the invasiveness of BT-K cell lines. The present study is the first to investigate SOLD1 expression in vitro, in trophoblastic cell lines. Our data suggested that SOLD1 is involved in the regulation of the trophoblast invasiveness. Therefore, SOLD1 may play an active and crucial role in mediating communication at the fetomaternal interface.
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影响因子：
4.8
作者：
GODKIN, JD;BAZER, FW;ROBERTS, RM
通讯作者：
ROBERTS, RM
影响因子：
2.2
作者：
Schlafer, DH;Fisher, PJ;Davies, CJ
通讯作者：
Davies, CJ
影响因子：
3.6
作者：
MATAMOROS, RA;CAAMANO, L;REIMERS, TJ
通讯作者：
REIMERS, TJ
影响因子：
2.2
作者：
Gootwine, E
通讯作者：
Gootwine, E
影响因子：
3.6
作者：
Green, JA;Xie, SC;Roberts, RM
通讯作者：
Roberts, RM