Comparison of venous and capillary differential leukocyte counts using a standard hematology analyzer and a novel microfluidic impedance cytometer.
Comparison of venous and capillary differential leukocyte counts using a standard hematology analyzer and a novel microfluidic impedance cytometer.
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DOI：
10.1371/journal.pone.0043702
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Morgan H
中科院分区：
文献类型：
--
作者：
Hollis VS;Holloway JA;Harris S;Spencer D;van Berkel C;Morgan H
毛细管采血已被确定为一种潜在的适合用于在护理点(POC)的全血细胞计数(FBC)的诊断测试的技术，最近已经强调了对该技术的需求。在这项研究中，我们评估了毛细血管血细胞计数的准确性，并评估了用于POC测试的微型细胞仪的潜力。使用标准血细胞分析仪测量和比较正常临床范围内的指刺(毛细血管)和静脉血样的白细胞分类计数。然后，通过将现场测量结果与标准分析仪获得的结果进行比较，测试了我们新型微流控阻抗细胞仪(MIC)的准确性。使用标准血细胞分析仪对指刺和静脉血样的测量之间的一致性很高，在平均分类白细胞计数之间没有观察到临床上相关的差异。MIC和标准分析仪在同一位置测量的一致性数据显示，由MIC确定的白细胞计数显著降低。这种系统性的低估在测量的(正常)浓度范围内是一致的，这表明可以应用内部校正因子。从指尖样本获得的不同的白细胞计数准确地反映了从静脉血中获得的白细胞计数，这证实了毛细血管采血用于FBC的POC测试的潜力。此外，这里展示的MIC设备为FBC的未来发展和用于患者护理现场的相关测试提供了一种现实的技术。
Capillary blood sampling has been identified as a potentially suitable technique for use in diagnostic testing of the full blood count (FBC) at the point-of-care (POC), for which a recent need has been highlighted. In this study we assess the accuracy of capillary blood counts and evaluate the potential of a miniaturized cytometer developed for POC testing. Differential leukocyte counts in the normal clinical range from fingerprick (capillary) and venous blood samples were measured and compared using a standard hematology analyzer. The accuracy of our novel microfluidic impedance cytometer (MIC) was then tested by comparing same-site measurements to those obtained with the standard analyzer. The concordance between measurements of fingerprick and venous blood samples using the standard hematology analyzer was high, with no clinically relevant differences observed between the mean differential leukocyte counts. Concordance data between the MIC and the standard analyzer on same-site measurements presented significantly lower leukocyte counts determined by the MIC. This systematic undercount was consistent across the measured (normal) concentration range, suggesting that an internal correction factor could be applied. Differential leukocyte counts obtained from fingerprick samples accurately reflect those from venous blood, which confirms the potential of capillary blood sampling for POC testing of the FBC. Furthermore, the MIC device demonstrated here presents a realistic technology for the future development of FBC and related tests for use at the site of patient care.
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DOI：
10.1111/j.1751-553x.2008.01050.x
发表时间：
2008-04-01
影响因子：
3
作者：
Briggs, C.;Carter, J.;Corrons, J. -L. Vives
通讯作者：
Corrons, J. -L. Vives
影响因子：
3.8
作者：
Jani, Ilesh V.;Sitoe, Nadia E.;Peter, Trevor F.
通讯作者：
Peter, Trevor F.
影响因子：
2.7
作者：
Pierelli, L.;Zennaro, F.;Mannella, E.
通讯作者：
Mannella, E.
影响因子：
2.8
作者：
Sun, Tao;van Berkel, Cees;Morgan, Hywel
通讯作者：
Morgan, Hywel
DOI：
10.1046/j.1365-2257.2001.00388.x
发表时间：
2001-06-01
期刊：
CLINICAL AND LABORATORY HAEMATOLOGY
影响因子：
--
作者：
Yang, ZW;Yang, SH;Tang, Z
通讯作者：
Tang, Z