Oxidative stress induced carbonylation in human plasma.
Oxidative stress induced carbonylation in human plasma.
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DOI：
10.1016/j.jprot.2011.07.014
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发表时间：
2011-10-19
影响因子：
3.3
通讯作者：
Regnier FE
中科院分区：
文献类型：
--
作者：
Madian AG;Diaz-Maldonado N;Gao Q;Regnier FE
The focus of this study was on the assessment of technology that might be of clinical utility in identification, quantification, characterization of carbonylation in human plasma proteins. Carbonylation is widely associated with oxidative stress diseases. Breast cancer patient samples were chosen as a stress positive case based on the fact that oxidative stress has been reported to be elevated in this disease. Measurements of 8-isoprostane in plasma confirmed that breast cancer patients in this study were indeed experiencing significant oxidative stress. Carbonyl groups in proteins from freshly drawn blood were derivatized with biotin hydrazide after which the samples were dialyzed and the biotinylated proteins subsequently selected, digested and labeled with iTRAQ™ heavy isotope coding reagent(s). Four hundred sixty proteins were identified and quantified, 95 of which changed 1.5 fold or more in concentration. Beyond confirming the utility of the analytical method, association of protein carbonylation was examined as well. Nearly one fourth of the selected proteins were of cytoplasmic, nuclear, or membrane origin. Analysis of the data by unbiased knowledge assembly methods indicated the most likely disease associated with the proteins was breast neoplasm. Pathway analysis showed the proteins which changed in carbonylation were strongly associated with Brca1, the breast cancer type-1 susceptibility protein. Pathway analysis indicated the major molecular functions of these proteins are defense, immunity and nucleic acid binding.
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影响因子：
18.2
作者：
Banfi, Cristina;Brioschi, Maura;Agostoni, PierGiuseppe
通讯作者：
Agostoni, PierGiuseppe
DOI：
10.1038/nrm2831
发表时间：
2010-02
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1016/s0006-291x(02)00420-5
发表时间：
2002-05-24
影响因子：
3.1
作者：
Choi, JG;Malakowsky, CA;Gracy, RW
通讯作者：
Gracy, RW
影响因子：
14.8
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.
影响因子：
7.4
作者：
Borisov, OV;Goshe, MB;Smith, RD
通讯作者：
Smith, RD