The Macronuclear Genome of Stentor coeruleus Reveals Tiny Introns in a Giant Cell.
The Macronuclear Genome of Stentor coeruleus Reveals Tiny Introns in a Giant Cell.
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DOI：
10.1016/j.cub.2016.12.057
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发表时间：
2017-02-20
期刊：
影响因子：
--
通讯作者：
Sood P
中科院分区：
文献类型：
--
作者：
Slabodnick MM;Ruby JG;Reiff SB;Swart EC;Gosai S;Prabakaran S;Witkowska E;Larue GE;Fisher S;Freeman RM Jr;Gunawardena J;Chu W;Stover NA;Gregory BD;Nowacki M;Derisi J;Roy SW;Marshall WF;Sood P
The giant, single-celled organism Stentor coeruleus has a long history as a model system for studying pattern formation and regeneration in single cells. Stentor (Figure 1A,B) is a heterotrichous ciliate distantly related to familiar ciliate models such as Tetrahymena or Paramecium. The primary distinguishing feature of Stentor is its incredible size: a single cell is 1 millimeter long. Early developmental biologists, including T.H. Morgan, were attracted to the system because of its regenerative abilities -- if large portions of a cell are surgically removed, the remnant reorganizes into a normal-looking but smaller cell with correct proportionality. These biologists were also drawn to Stentor because it exhibits a rich repertoire of behaviors, including light avoidance, mechanosensitive contraction, food selection, and even the ability to habituate to touch, a simple form of learning usually seen in higher organisms. While early microsurgical approaches demonstrated a startling array of regenerative and morphogenetic processes in this single-celled organism, Stentor was never developed as a molecular model system. We report the sequencing of the Stentor coeruleus macronuclear genome and reveal key features of the genome: First, we find that Stentor uses the standard genetic code, suggesting that ciliate specific genetic codes arose after Stentor branched from other ciliates. We also discover that ploidy correlates with Stentor’s cell size. Finally, in the Stentor genome, we discover the smallest spliceosomal introns reported for any species. The sequenced genome opens the door to molecular analysis of single-cell regeneration in Stentor. Stentor coeruleus is a giant single-celled organism that can regenerate after being cut in half. Slabodnick et al. describe the Stentor genome, a key tool for future experiments to understand regeneration in a single cell. The genome is unusual in that it contains extremely small introns.
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影响因子：
9.2
作者：
Losick, Vicki P.;Fox, Donald T.;Spradling, Allan C.
通讯作者：
Spradling, Allan C.
影响因子：
48
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.042700299
发表时间：
2002-03-19
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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