Polyploidization and cell fusion contribute to wound healing in the adult Drosophila epithelium.
Polyploidization and cell fusion contribute to wound healing in the adult Drosophila epithelium.
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DOI：
10.1016/j.cub.2013.09.029
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发表时间：
2013-11-18
期刊：
影响因子：
9.2
通讯作者：
Spradling, Allan C.
中科院分区：
文献类型：
--
作者：
Losick, Vicki P.;Fox, Donald T.;Spradling, Allan C.
在组织损伤后，重建上皮完整性和生物合成能力至关重要。成年果蝇腹部上皮为研究有丝分裂后二倍体细胞如何参与修复提供了一个有吸引力的新系统。成年果蝇表皮上的刺穿伤口最初通过形成黑色素痂闭合。我们发现伤口附近的上皮细胞融合形成一个巨大的合胞体，该合胞体将痂下的薄片重新上皮化受损部位。其他的大细胞通过内环的初始化和多倍体的形成，或者通过细胞融合而形成。Rac GTPase活性是合胞体形成所必需的，而Hippo信号效应体Yorkie调节多倍体和细胞融合。大细胞的形成在功能上很重要，因为当多倍体和融合都被阻断时，伤口不能再上皮化。我们的观察结果表明，损伤后失去的细胞质量可以被多倍体而不是有丝分裂增殖所取代。我们认为，由多倍体或细胞融合产生的大细胞是必不可少的，因为它们比二倍体细胞更能机械地稳定伤口，特别是那些含有永久性无细胞结构的伤口，如疤痕组织。
Re-establishing epithelial integrity and biosynthetic capacity is critically important following tissue damage. The adult Drosophila abdominal epithelium provides an attractive new system to address how post-mitotic diploid cells contribute to repair. Puncture wounds to the adult Drosophila epidermis close initially by forming a melanized scab. We found that epithelial cells near the wound site fuse to form a giant syncytium, which sends lamellae under the scab to re-epithelialize the damaged site. Other large cells arise more peripherally by initiating endocycles and becoming polyploid, or by cell fusion. Rac GTPase activity is needed for syncytium formation, while the Hippo signaling effector Yorkie modulates both polyploidization and cell fusion. Large cell formation is functionally important because when both polyploidization and fusion are blocked, wounds do not re-epithelialize. Our observations indicate that cell mass lost upon wounding can be replaced by polyploidization instead of mitotic proliferation. We propose that large cells generated by polyploidization or cell fusion are essential because they are better able than diploid cells to mechanically stabilize wounds, especially those containing permanent acellular structures, such as scar tissue.
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DOI：
10.1083/jcb.201304054
发表时间：
2013-06-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Machlus KR;Italiano JE Jr
通讯作者：
Italiano JE Jr
影响因子：
4.6
作者：
Fox, Donald T.;Duronio, Robert J.
通讯作者：
Duronio, Robert J.
影响因子：
3.3
作者：
Lesch, Christine;Jo, Juyeon;Galko, Michael J.
通讯作者：
Galko, Michael J.
影响因子：
56.9
作者：
Mace, KA;Pearson, JC;McGinnis, W
通讯作者：
McGinnis, W
影响因子：
8.8
作者：
Jenett, Arnim;Rubin, Gerald M.;Zugates, Christopher T.
通讯作者：
Zugates, Christopher T.