fgr proto-oncogene mRNA induced in B lymphocytes by Epstein–Barr virus infection
fgr proto-oncogene mRNA induced in B lymphocytes by Epstein–Barr virus infection
复制标题
EB 病毒感染 B 淋巴细胞中诱导的 fgr 原癌基因 mRNA
作者：
M. S. Cheah;T. Ley;S. Tronick;K. Robbins
几种急性转化逆转录病毒编码酪氨酸特异性蛋白激酶，这些蛋白激酶与某些生长因子的细胞表面受体具有结构和功能关系1，2。一种这样的酪氨酸激酶由Gardner-Rasheed猫肉瘤病毒（GR-FeSV）3-6的onc基因v-fgr编码。最近，我们已经分离并鉴定了与病毒onc序列相对应的人类基因c-fgr，并表明c-fgr是位于1号染色体短臂上的独特基因（参考文献7）。在这里，我们报告，某些淋巴瘤（但不是肉瘤或癌）表达fgr相关的信使RNA。该转录本在自然感染EB病毒（EBV）的伯基特淋巴瘤细胞系中检测到，但在EBV阴性伯基特淋巴瘤细胞中未检测到。通过EBV感染体外建立的正常脐带或外周血淋巴细胞系也含有可检测的c-fgr mRNA。此外，当用EBV故意感染未感染的伯基特淋巴瘤细胞系时，观察到稳态c-fgr mRNA浓度增加50倍。这些发现首次证明了DNA肿瘤病毒感染后原癌基因的诱导。
Several acute transforming retroviruses encode tyrosine-specific protein kinases which possess structural and functional relationships to cell-surface receptors for certain growth factors1,2. One such tyrosine kinase is encoded by the onc gene, v-fgr, of Gardner-Rasheed feline sarcoma virus (GR-FeSV)3–6. Recently, we have isolated and characterized the human gene, c-fgr, corresponding to the viral onc sequence and have shown that c-fgr is a unique gene located on the short arm of chromosome 1 (ref. 7). Here we report that certain lymphomas (but not sarcomas or carcinomas) express fgr-related messenger RNA. This transcript is detected in Burkitt's lymphoma cell lines naturally infected with Epstein-Barr virus (EBV), but not in EBV-negative Burkitt's lymphoma cells. Normal umbilical cord or peripheral blood lymphocyte lines established in vitro by EBV infection also contain detectable c-fgr mRNA. Moreover, a 50-fold increase of the steady-state c-fgr mRNA concentration is observed when uninfected Burkitt's lymphoma cell lines are deliberately infected with EBV. These findings demonstrate for the first time the induction of a proto-oncogene in response to infection by a DNA tumour virus.
DOI：
10.1073/pnas.79.24.7824
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DALLAFAVERA, R;BREGNI, M;CROCE, CM
通讯作者：
CROCE, CM
DOI：
10.1073/pnas.80.7.1987
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HENDERSON, A;RIPLEY, S;KIEFF, E
通讯作者：
KIEFF, E