Using buoyant mass to measure the growth of single cells.
Using buoyant mass to measure the growth of single cells.
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DOI:
10.1038/nmeth.1452
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发表时间:
2010-05
期刊:
影响因子:
48
通讯作者:
Manalis, Scott R.
中科院分区:
文献类型:
--
作者:
Godin, Michel;Delgado, Francisco Feijo;Son, Sungmin;Grover, William H.;Bryan, Andrea K.;Tzur, Amit;Jorgensen, Paul;Payer, Kris;Grossman, Alan D.;Kirschner, Marc W.;Manalis, Scott R.
We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the ‘instantaneous’ growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.
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影响因子:
3.2
作者:
KUBITSCHEK, HE
通讯作者:
KUBITSCHEK, HE
影响因子:
3.7
作者:
PRESCOTT, DM
通讯作者:
PRESCOTT, DM
影响因子:
4
作者:
Godin, Michel;Bryan, Andrea K.;Manalis, Scott R.
通讯作者:
Manalis, Scott R.
影响因子:
--
作者:
Conlon, Ian;Raff, Martin
通讯作者:
Raff, Martin
DOI:
10.1073/pnas.75.9.4384
发表时间:
1978-01-01
影响因子:
11.1
作者:
ELLIOTT, SG;MCLAUGHLIN, CS
通讯作者:
MCLAUGHLIN, CS