Nucleotide sequence of the nucleoprotein gene of the RC·HL strain of rabies virus, a seed strain used for animal vaccine production in Japan
Nucleotide sequence of the nucleoprotein gene of the RC·HL strain of rabies virus, a seed strain used for animal vaccine production in Japan
复制标题
日本用于动物疫苗生产的种子株狂犬病病毒RC·HL株核蛋白基因的核苷酸序列
作者：
H. Goto;N. Minamoto;Hiroshi Ito;M. Sugiyama;T. Kinjo;K. Mannen;K. Mifune;A. Kawai
利用噬菌体载体（λ ZAP Ⅱ）和从感染狂犬病病毒RC·HL株的IMR-32细胞中提取的mRNA，构建了一个cDNA文库。这些克隆含有约1.4 kb的cDNA插入片段，其中最长的开放阅读框与报道的CVS、PV和SAD B19三个固定菌株的N cDNA的长度相同。将RC·HL株的核苷酸序列和推导的氨基酸序列与三株进行比较，同源性分别为91.5-91.8%和95.1- 96.0%。在与3个毒株中至少一个毒株的相应位点不相同的183个核苷酸中，有41个核苷酸与CVS毒株相同，而与其他两个毒株中的任何一个毒株相同的核苷酸只有3个。在氨基酸序列中，我们发现了29个残基，这是不共享的所有四个菌株，其中11个是从根本上不同的氨基酸，可能会导致蛋白质的构象变化的取代，并且，此外，其中5个位于该区域靠近C端。RC·HL和CVS株间的氨基酸替换数较其他3株少。这些结果与RC·HL和CVS株来源于同一实验室巴斯德病毒株的假设并不矛盾。
By using a phage vector (lambda ZAP II) and the mRNA extracted from IMR-32 cells infected with the RC·HL strain of rabies virus, we constructed a cDNA library from which four nucleoprotein (N)-specific cDNA clones were obtained by Southern blot hybridization. These clones contained a cDNA insert of about 1.4 kb, in which the longest open reading frame was the same length as that reported for the N cDNA of three fixed strains, CVS, PV, and SAD B19. When the nucleotide and deduced amino acid sequences were compared between the RC·HL and the three strains, homology was within the range of 91.5–91.8% and 95.1–96.0%, respectively. Of 183 nucleotides of the RC·HL N-cDNA that were not identical to that of the corresponding site of at least one of the three strains, 41 were shared with the CVS strain, whereas only three were shared with either of the other two strains. In the amino acid sequence, we found 29 residues that were not shared in common with all of the four strains, 11 of which were the substitutions with radically different amino acids that might cause conformational changes of the protein, and, in addition, five of which were located in the region close to the C terminus. The number of such amino acid substitutions between the RC·HL and CVS strains was smaller than that of the other three strains. These results are not inconsistent with the presumption that the RC·HL and CVS strains originated from the same laboratory strain of the Pasteur viruses.
影响因子：
3.7
作者：
LEE, PWK;HAYES, EC;JOKLIK, WK
通讯作者：
JOKLIK, WK
DOI：
10.1073/pnas.84.24.9165
发表时间：
1987-12-01
影响因子：
11.1
作者：
DIETZSCHOLD, B;WANG, HH;KOPROWSKI, H
通讯作者：
KOPROWSKI, H