Dose- and time-dependent effects of collagenase clostridium histolyticum injection on transverse carpal ligament elastic modulus and thickness in vitro.
Dose- and time-dependent effects of collagenase clostridium histolyticum injection on transverse carpal ligament elastic modulus and thickness in vitro.
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DOI：
10.1371/journal.pone.0277187
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Li, Zong-Ming
中科院分区：
文献类型：
--
作者：
Hawk, Jocelyn L.;Daulat, Sohail R.;Margolis, David S.;Li, Zong-Ming
通过注射溶组织梭菌胶原酶（CCH）生化改变腕横韧带（TCL）的力学特性是腕管综合征的一种潜在治疗方法。本研究的目的是确定CCH注射液对TCL弹性模量和厚度的时间和剂量依赖性。本研究从尸体手部解剖了9个tcl。分别在TCL上5个点注射50U、100U、150U、200U、250U剂量的CCH。在注射后立即，以及注射后2、4、6、8和24小时，使用机器人辅助超声成像对每个注射点进行b型和横波弹性成像。在每个时间点测量每次CCH剂量的TCL厚度和平均横波速度。CCH剂量200U和250U分别使24h后横波速度降低18.70%和30.01% （p<0.05）。150U、200U和250U的CCH剂量在24 h后分别使TCL厚度降低7.28%、10.97%和14.92% （p<0.05）。我们的研究结果表明，注射CCH可能有效地降解TCL组织，注射后24小时内，高剂量的CCH导致更大的组织降解。
A potential treatment for carpal tunnel syndrome is to biochemically alter the mechanical properties of the transverse carpal ligament (TCL) through Collagenase Clostridium Histolyticum (CCH) injection. The purpose of this study was to determine the time- and dose-dependent effects of CCH injection on TCL elastic modulus and thickness. Nine TCLs were dissected from cadaveric hands for this study. CCH doses of 50U, 100U, 150U, 200U, and 250U were injected into five points on the TCL, respectively. B-mode and shear wave elastography images were taken of each injection point using robot-assisted ultrasound imaging immediately after injection, as well as 2, 4, 6, 8, and 24 hours after injection. TCL thickness and mean shear wave speed were measured for each CCH dose at each time point. CCH doses of 200U and 250U decreased shear wave speed by 18.70% and 30.01% (p<0.05), respectively, after 24 hours. CCH doses of 150U, 200U, and 250U decreased TCL thickness by 7.28%, 10.97%, and 14.92%, respectively, after 24 hours (p<0.05). Our findings suggest that CCH injection may be effective in degrading TCL tissue, with higher doses of CCH resulting in greater tissue degradation up to 24 hours after injection.
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